This book constitutes the thoroughly refereed post-workshop proceedings of the Second International Workshop on Modelling and Simulation for Autonomous Systems, MESAS 2015, held in Prague, Czech Republic, in April 2015. The 18 revised full papers included in the volume were carefully reviewed and selected from 33 submissions. They are organized in the following topical sections: state of the art and future of AS; MS experimental frameworks for AS; methods and algorithms for AS.