This book constitutes the proceedings of the 28th International Conference on Information Processing in Medical Imaging, IPMI 2023, which took place in San Carlos de Bariloche, Argentina, in June 2023. The 63 full papers presented in this volume were carefully reviewed and selected from 169 submissions. They were organized in topical sections as follows: biomarkers; brain connectomics; computer-aided diagnosis/surgery; domain adaptation; geometric deep learning; groupwise atlasing; harmonization; federated learning; image synthesis; image enhancement; multimodal learning; registration; segmentation; self supervised learning; surface analysis and segmentation.