This two-volume set LNCS16690 - 16691 constitutes the refereed proceedings of the 22nd International Symposium on Bioinformatics Research and Applications, ISBRA 2026, held in Macao, China, during July 22–24, 2026. The 61 full papers included in these volumes were carefully reviewed and selected from 168 submissions. This year’s symposium brought together leading researchers, scientists, and industry professionals from around the world to share cutting-edge advancements, foster collaboration, and explore the future of bioinformatics and computational biology.