This book constitutes the thoroughly refereed post-conference proceedings of the second International Workshop on Interoperability and Open-Source Solutions for the Internet of Things, InterOSS-IoT 2016, held in Stuttgart, Germany, November 7, 2016. The 11 revised full papers presented were carefully reviewed and selected from 17 submissions during two rounds of reviewing. They are organized in topical sections on semantic interoperability, interoperabile architectures and platforms, business models and security, platform performance and applications.