This book constitutes the refereed proceedings of the 25th International Symposium on Model Checking Software, SPIN 2018, held in Malaga, Spain, in June 2018. The 14 papers presented, 1 short paper, and 1 demo-tool paper, were carefully reviewed and selected from 28 submissions. Topics covered include formal verification techniques for automated analysis of software; formal analysis for modeling languages, such as UML/state charts; formal specification languages, temporal logic, design-by-contract; model checking, automated theorem proving, including SAT and SMT; verifying compilers; abstraction and symbolic execution techniques; and much more.