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Mitochondrial Medicine: Volume 1: Targeting Mitochondria de Volkmar Weissig

Mitochondrial Medicine: Volume 1: Targeting Mitochondria: Methods in Molecular Biology, cartea 2275

Editat de Volkmar Weissig, Marvin Edeas
en Limba Engleză Hardback – 13 iun 2021
This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume I guides readers through chapters on QSAR models, DQAsomes, synthesis of Triphenylphosphonium Phospholipid Conjugates, testing of novel isomeric mitochondriotropic derivatives, mTRIP, mitochondria-targeted imaging nanoplatforms, live-cell assessment, mitochondrial coenzyme Q10, rat liver Mitochondrial Lipidome, and Mito-SinCe2. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and accessible, Mitochondrial Medicine, Second Edition, Volume 1: Targeting Mitochondria aims to be a comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine.
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Specificații

ISBN-13: 9781071612613
ISBN-10: 1071612611
Pagini: 459
Ilustrații: XV, 459 p. 128 illus., 72 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 1 kg
Ediția:2nd ed. 2021
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Using QSAR Models to Predict Mitochondrial Targeting by Small Molecule Xenobiotics within Living Cells.- DQAsomes as the Prototype of Mitochondria-targeted Pharmaceutical Nanocarriers: An Update.- Synthesis and Characterization of Mitochondria-Targeted Triphenyltriphenylphosphonium Bolaamphiphiles.- Synthesis and Evaluation of 18F-labeled Fluoroalkyl Triphenylphosphonium Salts as Mitochondrial Voltage Sensors in PET Myocardial Imaging.- Novel Mitochondria-Targeted Triphenylphosphonium Conjugates of Linear beta-Phosphorylated Nitrones: Preparation, 31P NMR Mitochondrial Distribution, EPR Spin Trapping Reporting, and Site-Directed Anti-Apoptotic Properties.- Insights on Targeting Small Molecules to the Mitochondrial Matrix and the Preparation of MitoB and MitoP as exomarkers of Mitochondrial Hydrogen Peroxide.- Synthesis of Triphenylphosphonium Phospholipid Conjugates for the Preparation of Mitochondriotropic Liposomes.- Imaging Mitochondrial Hydrogen Peroxide in Living Cells.- Synthesis and Testing Of Novel Isomeric Mitochondriotropic Derivatives of Resveratrol and Quercetin.- Bridging the Gap between Nature and Antioxidant Setbacks: Delivering Gallic Acid to Mitochondria.- Liposomal Delivery of Cyclocreatine Impairs Cancer Cell Bioenergetics Mediating Apoptosis.- Mt-fura-2, a ratiometric Mitochondria-targeted Ca2+ sensor. Determination of spectroscopic properties and Ca2+ Imaging Assays.- Sequence-Specific Control of Mitochondrial Gene Transcription using Programmable Synthetic Gene Switches called MITO-PIPs.- Targeting the Mitochondrial Genome via a MITO-Porter: Evaluation of mtDNA and mtRNA Levels and Mitochondrial Function.- mTRIP, an Imaging Tool to Investigate Mitochondrial DNA dynamics in Physiology and Disease at the Single-Cell Resolution.- Development of Mitochondria-Targeted Imaging Nanoplatforms by Incorporation of Fluorescent Carbon Quantum Dots.- Norbormide-based Probes and their Application for Mitochondrial Imaging in Drosophila Melanogaster.- Live-cell Assessment of Reactive Oxygen Species Levels Using Dihydroethidine.- Protein Supercomplex Recording In Living Cells Via Position-Specific Fluorescence Lifetime Sensors.- Identification of Peroxynitrite by Profiling Oxidation and Nitration Products from Mitochondria-Targeted Arylboronic Acid.- Mitochondrial Coenzyme Q10 Determination via Isotope Dilution Liquid Chromatography Tandem Mass Spectrometry.- Janus-type Mesoporous Silica Nanoparticles for Sequential Tumoral Cell and Mitochondria Targeting.- Split Green Fluorescent Protein-Based Contact Site Sensor (SPLICS) for Heterotypic Organelle Juxtaposition as Applied to ER – Mitochondria Proximities.- Qualitative Characterization of the Rat liver Mitochondrial Lipidome using All Ion Fragmentation on an Exactive Benchtop Orbitrap MS.- Live Imaging of Mitochondria in Kidney Tissue.- Time-resolved Imaging of Mitochondrial Flavin Fluorescence and Its Applications for Evaluating the Oxidative State in Living Cardiac Cells.- Mito-SinCe2 Approach to Analyze Mitochondrial Structure-Function In Single Cells.- Computer-aided Prediction of Protein Mitochondrial Localization.

Textul de pe ultima copertă

This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume I guides readers through chapters on QSAR models, DQAsomes, synthesis of Triphenylphosphonium Phospholipid Conjugates, testing of novel isomeric mitochondriotropic derivatives, mTRIP, mitochondria-targeted imaging nanoplatforms, live-cell assessment, mitochondrial coenzyme Q10, rat liver Mitochondrial Lipidome, and Mito-SinCe2. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and accessible, Mitochondrial Medicine, Second Edition, Volume 1: Targeting Mitochondria aims to be a comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts