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Isolation of Plant Organelles and Structures: Methods and Protocols (Methods in Molecular Biology, nr. 1511)

Editat de Nicolas L. Taylor, A. Harvey Millar
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en Limba Engleză Carte Hardback – 12 Oct 2016
This book brings together the major techniques used in the isolation or enrichment of individual populations of organelles and other subcellular structures from plants with the goal that, by being able to isolate subcellular structures, the research and understanding of various facets of compartmentalized function in plant cells can be advanced.
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Specificații

ISBN-13: 9781493965311
ISBN-10: 149396531X
Pagini: 300
Dimensiuni: 178 x 254 x 21 mm
Greutate: 0.85 kg
Ediția: 1st ed. 2017
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării: New York, NY, United States

Cuprins

1. The Isolation of Plant Organelles and Structures in the Post-Genomics Era
            A. Harvey Millar and Nicolas L. Taylor
 
2. Approaches to Characterize Organelle, Compartment, or Structure Purity
            Stefanie J. Mueller, Sebastian N.W. Hoernstein, and Ralf Reski
 
Part I: Isolation of Organelles and Organelle Compartments
<  3. Isolation of Nuclei and Nucleoli
            Alison F. Pendle and Peter J. Shaw
 
4. Isolation and Suborganellar Fractionation of Arabidopsis Chloroplasts
            Úrsula Flores-Pérez and Paul Jarvis
 
5. Isolation of Chromoplasts and Sub-Organellar Compartments from Tomato and Bell Pepper Fruit
            Cristina Barsan, Marcel Kuntz, and Jean-Claude Pech  
6. Leucoplast Isolation and Subfractionation
            William C. Plaxton
 
7. Isolation of Mitochondria, Their Sub-Organellar Compartments, and Membranes
            Owen Duncan, A. Harvey Millar, and Nicolas L. Taylor
 
8. Isolation of Arabidopsis Leaf Peroxisomes and the Peroxisomal Membrane
            Sigrun Reumann and Piotr Lisik
 
9. Isolation of Vacuoles and the Tonoplast
            Jan Zouhar
 
10. Isolation of Endoplasmic Reticulum and Its Membrane
            G. Eric Schaller
 
11. Enrichment of Golgi Membranes from Triticum aestivum (Wheat) Seedlings
            Wei Zeng, Berit Ebert, Harriet T. Parsons, Carsten Rautengarten, Antony Bacic, and Joshua L. Heazlewood
 
12. Isolation of Autolysosomes from Tobacco BY-2 Cells
            Chihiro Takatsuka, Yuko Inoue, and Yuji Moriyasu
 
13. Isolation of Protein Storage Vacuoles and Their Membranes
            Tomoo Shimada and Ikuko Hara-Nishimura
 
Part II: Isolation of Other Structures and Compartments
 <
14. Isolation of the Cell Wall
            Hervé Canut, Cécile Albenne, and Elisabeth Jamet
 
15. Isolation of Plasmodesmata
            Christine Faulkner and Emmanuelle M.F. Bayer
 
16. Isolation of Plasma Membrane and Plasma Membrane Microdomains
            Anzu Minami, Daisuke Takahashi, Yukio Kawamura, and Matsuo Uemura
 
17. Enrichment of the Plant Cytosolic Fraction
            Jeemeng Lao, Andreia M. Smith-Moritz, Jennifer C. Mortimer, and Joshua L. Heazlewood  
18. Isolation of Apoplast
            Erik Andreasson, Kibrom B. Abreha, and Svante Resjö
 
19. Isolation of Cytosolic Ribosomes
            Hanna Klang Årstrand and Maria V. Turkina
 
20. Isolation of Plastid Ribosomes
            Kenichi Yamaguchi
 
21. Isolation of Mitochondrial Ribosomes
            Adam J. Carroll
 
22. Isolation of Microtubules and Microtubule-Associated Proteins
            Takahiro Hamada and Seiji Sonobe
 
23. Isolation of Actin and Actin Binding Proteins
            Etsuo Yokota  
24. Purification of 26S Proteasomes and Their Sub-Complexes from Plants
            Richard S. Marshall, David C. Gemperline, and Richard D. Vierstra

Caracteristici

Includes cutting-edge methods and protocols for the study of plant organelles and structures
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts

Textul de pe ultima copertă

This book brings together the major techniques used in the isolation or enrichment of individual populations of organelles and other subcellular structures from plants with the goal that, by being able to isolate subcellular structures, the research and understanding of various facets of compartmentalized function in plant cells can be advanced. Written for the highly successful Methods in Molecular Biology series, expert contributors provide chapters that contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Isolation of Plant Organelles and Structures: Methods and Protocols will greatly aid those who regularly isolate subcellular components as well as those whose research has lead them to focus on a subcellular compartment or a particular process for the first time, thus producing the need to be able to isolate it or enrich it for study.