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Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Prokaryotic and Eukaryotic Systems

David M. Chenoweth
en Limba Engleză Hardback – 15 mai 2020
Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Prokaryotic and Eukaryotic Systems, Volume 638, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Sample chapters from this new release include In vitro characterization of the colibactin-activating peptidase ClbP enables development of a fluorogenic activity probe, Using FDAA probes to study cell division in Bacillus subtilis, Chemoenzymatic synthesis of UDP-sugars, Chemical tools for selective activity profiling of bacterial penicillin-binding proteins, Chemical Probes Reveal and Extraseptal Mode of Cross-linking in Staphylococcus Aureus, and much more.


  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Includes the latest information on retinoid signaling pathways
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Specificații

ISBN-13: 9780128201459
ISBN-10: 0128201452
Pagini: 358
Dimensiuni: 152 x 229 mm
Greutate: 0.65 kg
Editura: ELSEVIER SCIENCE

Public țintă

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.

Cuprins

    1. Utility of bacterial peptidoglycan recycling enzymes in the chemoenzymatic synthesis of valuable UDP sugar substratesOphelia I. Ukaegbu, Kristen E. DeMeester, Hai Liang, Ashley R. Brown, Zachary S. Jones and Catherine Leimkuhler Grimes
    2. Chemical tools for selective activity profiling of bacterial penicillin-binding proteinsShabnam Sharifzadeh, Nathaniel W. Brown, Joshua D. Shirley, Kevin E. Bruce, Malcolm E. Winkler and Erin E. Carlson
    3. Fluorescent stem peptide mimics: In situ probes for peptidoglycan crosslinkingSamir Gautam, Taehan Kim, Rebecca Howell and David A. Spiegel
    4. Gram-scale preparation of the antibiotic lead compound salicyl-AMS, a potent inhibitor of bacterial salicylate adenylation enzymesNihar Kinarivala, Lisa Standke, Tezcan Guney, Cheng Ji, Naoyoshi Noguchi, Yasutomi Asano and Derek S. Tan
    5. Probe-enabled approaches for function-dependent cell sorting and characterization of microbiome subpopulationsAndrea K. Steiger, Sarah J. Fansler, Christopher Whidbey and Aaron T. Wright
    6. Biochemical analysis of NlpC/p60 peptidoglycan hydrolase activityByungchul Kim, Juliel Espinosa and Howard C. Hang
    7. Controlled release of bioactive signaling molecules using DNA-icosahedronMaulik S. Jani, Aneesh T. Veetil and Yamuna Krishnan
    8. Imaging nascent transcription in wholemount vertebrate embryos to characterize zygotic genome activationHui Chen and Matthew C. Good
    9. Engineering reversible cell-cell interactions with chemical biologyClifford M. Csizmar and Carston R. Wagner
    10. Fast phosphine-activated control of protein function using unnatural lysine analoguesJoshua S. Wesalo and Alexander Deiters
    11. Photopharmacological control of lipid functionJohannes Morstein and Dirk Trauner
    12. Expanding the substrate selectivity in SNAP/CLIP-tagging of intracellular targetsMiguel Macias-Contreras, Kevin N. Little and Lei Zhu
    13. Light-induced protein proximity by activation of gibberellic acid derivatives in living cellsMichael J. Ziegler and Richard Wombacher
    14. Photoactivatable trimethoprim-based probes for spatiotemporal control of biological processesDaniel Z. Wu, Michael A. Lampson and David M. Chenoweth
    15. Site-specific antibody fragment conjugates for targeted imagingRobert Maloney, Zakey Yusuf Buuh, Yue Zhao and Rongsheng E. Wang
    16. Quantifying protein-protein interactions of the acyl carrier protein with solvatochromic probes
Katherine Charov and Michael D. Burkart