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Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation de David M. Chenoweth

Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation: Methods in Enzymology, cartea 639

David M. Chenoweth
en Limba Engleză Hardback – 31 mai 2020
Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation, Volume 639, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Fluorogenic detection of protein aggregates in live cells using the AggTag method, Synthesis and Application of Ratiometric Probes for Hydrogen Peroxide Detection, Chemical Tools for Multicolor Protein FRET with Tryptophan, Fluorescing Isofunctional Ribonucleosides for Adenosine Deaminase Activity and Inhibition, Temporal profiling establishes a dynamic S-palmitoylation cycle, Solvation-guided design of fluorescent probes for discrimination of amyloids, and much more.


  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Includes the latest information on retinoid signaling pathways
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Specificații

ISBN-13: 9780128211519
ISBN-10: 0128211512
Pagini: 430
Dimensiuni: 152 x 229 mm
Greutate: 0.74 kg
Editura: ELSEVIER SCIENCE
Seria Methods in Enzymology

Public țintă

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.

Cuprins

    1. Fluorogenic detection of protein aggregates in live cells using the AggTag methodKwan Ho Jung and Xin Zhang
    2. Synthesis and application of a ratiometric probe for hydrogen peroxideRuwen Yin, Yuan Fang, Xinqi Zhou and Cliff I. Stains
    3. Protein labeling for FRET with methoxycoumarin and acridonylalanineChloe M. Jones, Yarra Venkatesh and E. James Petersson
    4. Ascertaining the activity and inhibition of adenosine deaminase via fluorescence-based assaysPaul T. Ludford III and Yitzhak Tor
    5. Method to discriminate amyloids using fluorescent probesKristine L. Teppang, Rachel S. Ehrlich and Jerry Yang
    6. Chemical modulation of circadian rhythms and assessment of cellular behavior via indirubin and derivativesHui-Hsien Lin, Kelly L. Robertson, Sujeewa S. Lellupitiyage Don, Stephanie R. Taylor and Michelle E. Farkas
    7. Methods for analysis of near-infrared (NIR) quenched-fluorescent contrast agents in mouse models of cancerJohn C. Widen, Martina Tholen, Joshua J. Yim and Matthew Bogyo
    8. Acid-brightening fluorescent protein (abFP) for imaging acidic vesicles and organellesNanxi Wang and Lei Wang
    9. 4-Cyanoindole-based fluorophores for biological spectroscopy and microscopyArusha Acharyya, Ismail A. Ahmed and Feng Gai
    10. Dissecting contributions of catalytic and reader domains in regulation of histone demethylationNektaria Petronikolou, James E. Longbotham and Danica Galonić Fujimori
    11. Ortho-Phthalaldehyde (OPA)-based chemoselective protein bioconjugation and peptide cyclizationQing Zhang, Yue Zhang and Xuechen Li
    12. Chemical synthesis of di-ubiquitin modified histones for further biochemical studiesJia-Bin Li, Jun Liang and Changlin Tian
    13. Chemical probes and methods for single-cell detection and quantification of epichaperomes in hematologic malignanciesSwathi Merugu, Sahil Sharma, Justin Kaner, Chander Digwal, Mayumi Sugita, Suhasini Joshi, Tony Taldone, Monica L. Guzman and Gabriela Chiosis
    14. FlICk (fluorescent isoindole crosslinking) for peptide staplingMihajlo Todorovic and David M. Perrin
    15. Selective protein N-terminal labeling with N-hydroxysuccinimide estersHanjie Jiang, Gabriel D. D'Agostino, Philip A. Cole and Daniel R. Dempsey
    16. Covalent live-cell labeling of proteins using a photoreactive fluorogenTewoderos M. Ayele, Steve D. Knutson and Jennifer M. Heemstra
    17. Photoconvertible diazaxanthilidene dyes for live cell imagingRachel M. Lackner, Christopher L. Johnny and David M. Chenoweth
    18. No-wash live cell imaging with a photoswitchable near-infrared hCRBPII protein-fluorophore tag
Wei Sheng, James H. Geiger and Babak Borhan