This second edition volume expands on the previous edition with updates on the latest innovative techniques and protocols used to study phloem. The chapters in this book cover the whole spectrum of scientific techniques from molecular biology to anatomical studies; imaging and tracer-based analyses; theoretical and modeling approaches; and the whole range of functional aspects of the phloem, from molecular-scale processes to its role in whole plant performance and responses to stress. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Phloem: Methods and Protocols, Second Edition is a practical and detailed reference for scientists with different backgrounds. This volume provides enough context of methodological principles to help researchers select the most appropriate methods for their specific questions, and will inspire them to combine techniques from different subfields of phloem studies to learn more about this sensitive and complex tissue.