This detailed volume explores quantum dot (QD) techniques, spanning from materials preparation and surface functionalization to bioimaging and sensing. Split into four sections, the book covers the preparation of different types of QDs, including binary and ternary compositions, tutorials on using computational tools to optimize optical properties and enable single-QD tracking, the functionalization of QDs for bioimaging and cellular labeling, as well as the application of QDs for biosensing and biomarker detection. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Up-to-date and practical, Quantum Dots: Applications in Biology, Fourth Edition serves as a bridge between fundamental research on QDs and impactful applications in the life sciences.