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Laboratory Methods in Enzymology: Protein Part C de Jon Lorsch

Laboratory Methods in Enzymology: Protein Part C: Methods in Enzymology, cartea 541

Jon Lorsch
en Limba Engleză Hardback – 9 iun 2014
In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts.


  • Indispensable tool for the researcher
  • Carefully written and edited by experts to contain step-by-step protocols
  • In this volume we have brought together a number of core protocols concentrating on Protein
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Specificații

ISBN-13: 9780124201194
ISBN-10: 0124201199
Pagini: 296
Dimensiuni: 152 x 229 x 20 mm
Greutate: 0.59 kg
Ediția:New.
Editura: ELSEVIER SCIENCE
Seria Methods in Enzymology

Public țintă

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Cuprins

TCA PrecipitationCoimmunoprecipitation of Proteins from YeastCoupling Antibody to Cyanogen Bromide-Activated SepharoseAnalysis of Protein–Protein Interactions by CoimmunoprecipitationUse and Application of Hydrophobic Interaction Chromatography for Protein PurificationHydroxyapatite Chromatography: Purification Strategies for Recombinant ProteinsSalting out of Proteins Using Ammonium Sulfate PrecipitationUsing Ion Exchange Chromatography to Purify a Recombinantly Expressed ProteinGel Filtration Chromatography (Size Exclusion Chromatography) of ProteinsExpression and Purification of Membrane ProteinsExplanatory Chapter: Choosing the Right DetergentOne-dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE)Coomassie Blue StainingSilver Staining of SDS-polyacrylamide GelStandard In Vitro Assays for Protein–Nucleic Acid Interactions – Gel shift Assays for RNA and DNA BindingProtein Filter BindingExplanatory Chapter: Troubleshooting recombinant protein expression: generalExplanatory Chapter: Troubleshooting protein expression: what to do when the protein is not solubleWestern Blotting using Chemiluminescent Substrates