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Laboratory Methods in Enzymology: Protein Part B de Jon Lorsch

Laboratory Methods in Enzymology: Protein Part B: Methods in Enzymology, cartea 539

Jon Lorsch
en Limba Engleză Hardback – 29 apr 2014
Laboratory Methods in Enzymology: Protein Part B brings together a number of core protocols concentrating on protein, carefully written and edited by experts.

  • Indispensable tool for the researcher
  • Carefully written and edited by experts to contain step-by-step protocols
  • In this volume we have brought together a number of core protocols concentrating on protein
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Specificații

ISBN-13: 9780124201200
ISBN-10: 0124201202
Pagini: 240
Dimensiuni: 152 x 229 x 14 mm
Greutate: 0.49 kg
Ediția:New.
Editura: ELSEVIER SCIENCE
Seria Methods in Enzymology

Public țintă

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Cuprins

Small-scale expression of proteins in E. coli
Protein expression-Yeast
Recombinant protein expression in baculovirus-infected insect cells
Single cell cloning of a stable mammalian cell line
In vitro synthesis of proteins in bacterial extracts
Preparation of a Saccharomyces cerevisiae cell-free extract for in vitro translation
Yeast two-hybrid screen
UV cross-linking of interacting RNA and protein in cultured cells
Analysis of RNA–protein interactions by cell mixing
General protein–protein cross-linking
Chromatin immunoprecipitation and multiplex sequencing (ChIP-Seq) to identify global transcription factor binding sites in the nematode Caenorhabditis elegans
PAR-CLIP (photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation): A step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins
Determining the RNA specificity and targets of RNA-binding proteins using a three-hybrid system
Dissecting a known RNA–protein interaction using a yeast three-hybrid system
Identifying proteins that bind a known RNA sequence using the yeast three-hybrid system