Electron Microscopy: Methods and Protocols (Methods in Molecular Biology, nr. 1117)
Editat de John Kuoen Limba Engleză Carte Hardback – 20 Dec 2013
Authoritative and practical, Electron Microscopy: Methods and Protocols, Third Edition provides the most up-to-date and essential information in electron microscopy techniques and methods provided in this edition will assist in advancing future molecular and biological research.
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Specificații
ISBN-13: 9781627037754
ISBN-10: 1627037756
Pagini: 750
Ilustrații: 261 illus., 24 illus. in color., 237 schwarz-weiße und 24 farbige Abbildungen, 22 schwarz-weiße Tabellen
Dimensiuni: 178 x 254 x 50 mm
Greutate: 1.65 kg
Ediția: 3rd ed. 2014
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării: Totowa, NJ, United States
ISBN-10: 1627037756
Pagini: 750
Ilustrații: 261 illus., 24 illus. in color., 237 schwarz-weiße und 24 farbige Abbildungen, 22 schwarz-weiße Tabellen
Dimensiuni: 178 x 254 x 50 mm
Greutate: 1.65 kg
Ediția: 3rd ed. 2014
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării: Totowa, NJ, United States
Public țintă
Professional/practitionerCuprins
1.
Conventional
Specimen
Preparation
Techniques
For
Transmission
Electron
Microscopy
of
Cultured
Cells
John J. Bozzola
2. Microwave-assisted Processing and Embedding for Transmission Electron Microscopy
Paul Webster
3. Processing Plant Tissues for Ultrastructural Study John Kuo
4. Staining Sectioned Biological Specimens for Transmission Electron Microscopy:
Conventional and En Bloc Stains
E. Ann Ellis
5. Metal Shadowing for Electron Microscopy
Gregory M. Hendricks
6. Freeze Fracture and Freeze Etching
Douglas E. Chandler and William P. Sharp
7. Conventional Specimen Preparation Techniques For Scanning Electron Microscopy of Biological Specimens
John J. Bozzola
8. High-pressure Freezing: Current State and Future Prospects
Andres Kaech and Urs Ziegler
9. Cryo-fixation by Self-pressurized Rapid Freezing Markus Grabenbauer, Hong-Mei Han, and Jan Huebinger
10. Cryo-Electron Microscopy of Vitreous Sections
Petr Chlanda and Martin Sachse
11. Negative Staining and Cryo-negative Staining: Applications in Biology and Medicine
J. Robin Harris
12. Electron Microscopy of the Microtubule Cytoskeleton Assembly Vitro
Margaret Coughlin, Aaron C. Groen, Timothy J. Mitchison
13. Cryosectioning Fixed and Cryoprotected Biological Material for Immmunocytochemistry
Paul Webster and Alexandre Webster
14. Analysis of Specificity in Immunoelectron Microscopy
Christian Hacker and John M. Lucocq
15. Cryo-Electron Microscopy of Membrane Proteins
Kenneth N. Goldie, Priyanka Abeyrathne, Fabian Kebbel, Mohamed Chami, Philippe Ringler, and Henning Stahlberg
16. Tracking DNA and RNA Sequences at High Resolution
Dušan Cmarko, Anna Ligasová, and Karel Koberna
17. Visualization of DNA and Protein-DNA Complexes with Atomic Force Microscopy
Yuri L. Lyubchenko, Alexander A. Gall, and Luda S. Shlyakhtenko
18. Biological Applications of Phase-Contrast Electron Microscopy
Kuniaki Nagayama
19. Single Particle Cryo-Electron Microscopy And 3-D Reconstruction Of Viruses
Fei Guo and Wen Jiang
20. Electron Tomography for Organelles, Cells and Tissues
Wanzhong He and Yongning He
21. Correlative Light and Electron Microscopy: from Live Cell Dynamic to 3D Ultrastructure
Coralie Spiegelhalter, Jocelyn F. Laporte, and Yannick Schwab
22. Nanometer-resolution Fluorescence Electron Microscopy (Nano-EM) in Cultured Cells
Shigeki Watanabe, Martin Lehmann, Edward Hujber, Richard D. Fetter, Jackson Richards, Berit Söhl-Kielczynski, Annegret Felies, Christian Rosenmund, Jan Schmoranzer, and Erik M. Jorgensen
23. Correlative Fluorescence- and Electron Microscopy of Quantum Dot Labeled Proteins on Whole Cells in Liquid
Diana B. Peckys, Madeline J. Dukes, and Niels de Jonge
24. FIB-SEM Tomography in Biology
Caroline Kizilyaprak
25. Correlative Light and Electron Microscopy using Immunolabeled Sections
Heinz Schwarz and Bruno M. Humbel
26. Correlative 3D Imaging: CLSM and FIB-SEM Tomography Using High-Pressure Frozen, Freeze-Substituted Biological Samples
Miriam S. Lucas, Maja Guenthert, Philippe Gasser, Falk Lucas and Roger Wepf
27. Three-dimensional Imaging of Adherent Cells using FIB-SEM and STEM
Clarissa Villinger, Martin Schauflinger, Heiko Gregorius, Christine Kranz, Katharina Höhn, Soufi Nafeey, Paul Walther
28. X-Ray Microanalysis in the Scanning Electron Microscope Godfried M. Roomans and Anca Dragomir
29. Application of SEM and EDX in Studying Biomineralization in Plant Tissues
Honghua He
30. Freeze Stabilization and Cryopreparation Technique for Visualizing the Water Distribution in Woody Tissues by X-ray Imaging and Cryoscanning Electron Microscopy
Yasuhiro Utsumi and Yuzou Sano
31. Biological Applications of Energy-Filtered TEM
Martin Saunders and Jeremy A. Shaw
32. Secondary Ion Mass Spectrometry Imaging of Biological Cells and Tissues
Nicholas P. Lockyer
33. Elemental and Isotopic Imaging of Biological Samples using NanoSIMS
Matt R. Kilburn and Peta L. Clode
34. 3D Chemical Mapping: Application of Scanning Transmission (soft) X-ray Microscopy (STXM) in Combination with Angle-Scan Tomography in Bio-, Geo- and Environmental Science
Gregor Schmid and Martin Obst.
John J. Bozzola
2. Microwave-assisted Processing and Embedding for Transmission Electron Microscopy
Paul Webster
3. Processing Plant Tissues for Ultrastructural Study John Kuo
4. Staining Sectioned Biological Specimens for Transmission Electron Microscopy:
Conventional and En Bloc Stains
E. Ann Ellis
5. Metal Shadowing for Electron Microscopy
Gregory M. Hendricks
6. Freeze Fracture and Freeze Etching
Douglas E. Chandler and William P. Sharp
7. Conventional Specimen Preparation Techniques For Scanning Electron Microscopy of Biological Specimens
John J. Bozzola
8. High-pressure Freezing: Current State and Future Prospects
Andres Kaech and Urs Ziegler
9. Cryo-fixation by Self-pressurized Rapid Freezing Markus Grabenbauer, Hong-Mei Han, and Jan Huebinger
10. Cryo-Electron Microscopy of Vitreous Sections
Petr Chlanda and Martin Sachse
11. Negative Staining and Cryo-negative Staining: Applications in Biology and Medicine
J. Robin Harris
12. Electron Microscopy of the Microtubule Cytoskeleton Assembly Vitro
Margaret Coughlin, Aaron C. Groen, Timothy J. Mitchison
13. Cryosectioning Fixed and Cryoprotected Biological Material for Immmunocytochemistry
Paul Webster and Alexandre Webster
14. Analysis of Specificity in Immunoelectron Microscopy
Christian Hacker and John M. Lucocq
15. Cryo-Electron Microscopy of Membrane Proteins
Kenneth N. Goldie, Priyanka Abeyrathne, Fabian Kebbel, Mohamed Chami, Philippe Ringler, and Henning Stahlberg
16. Tracking DNA and RNA Sequences at High Resolution
Dušan Cmarko, Anna Ligasová, and Karel Koberna
17. Visualization of DNA and Protein-DNA Complexes with Atomic Force Microscopy
Yuri L. Lyubchenko, Alexander A. Gall, and Luda S. Shlyakhtenko
18. Biological Applications of Phase-Contrast Electron Microscopy
Kuniaki Nagayama
19. Single Particle Cryo-Electron Microscopy And 3-D Reconstruction Of Viruses
Fei Guo and Wen Jiang
20. Electron Tomography for Organelles, Cells and Tissues
Wanzhong He and Yongning He
21. Correlative Light and Electron Microscopy: from Live Cell Dynamic to 3D Ultrastructure
Coralie Spiegelhalter, Jocelyn F. Laporte, and Yannick Schwab
22. Nanometer-resolution Fluorescence Electron Microscopy (Nano-EM) in Cultured Cells
Shigeki Watanabe, Martin Lehmann, Edward Hujber, Richard D. Fetter, Jackson Richards, Berit Söhl-Kielczynski, Annegret Felies, Christian Rosenmund, Jan Schmoranzer, and Erik M. Jorgensen
23. Correlative Fluorescence- and Electron Microscopy of Quantum Dot Labeled Proteins on Whole Cells in Liquid
Diana B. Peckys, Madeline J. Dukes, and Niels de Jonge
24. FIB-SEM Tomography in Biology
Caroline Kizilyaprak
25. Correlative Light and Electron Microscopy using Immunolabeled Sections
Heinz Schwarz and Bruno M. Humbel
26. Correlative 3D Imaging: CLSM and FIB-SEM Tomography Using High-Pressure Frozen, Freeze-Substituted Biological Samples
Miriam S. Lucas, Maja Guenthert, Philippe Gasser, Falk Lucas and Roger Wepf
27. Three-dimensional Imaging of Adherent Cells using FIB-SEM and STEM
Clarissa Villinger, Martin Schauflinger, Heiko Gregorius, Christine Kranz, Katharina Höhn, Soufi Nafeey, Paul Walther
28. X-Ray Microanalysis in the Scanning Electron Microscope Godfried M. Roomans and Anca Dragomir
29. Application of SEM and EDX in Studying Biomineralization in Plant Tissues
Honghua He
30. Freeze Stabilization and Cryopreparation Technique for Visualizing the Water Distribution in Woody Tissues by X-ray Imaging and Cryoscanning Electron Microscopy
Yasuhiro Utsumi and Yuzou Sano
31. Biological Applications of Energy-Filtered TEM
Martin Saunders and Jeremy A. Shaw
32. Secondary Ion Mass Spectrometry Imaging of Biological Cells and Tissues
Nicholas P. Lockyer
33. Elemental and Isotopic Imaging of Biological Samples using NanoSIMS
Matt R. Kilburn and Peta L. Clode
34. 3D Chemical Mapping: Application of Scanning Transmission (soft) X-ray Microscopy (STXM) in Combination with Angle-Scan Tomography in Bio-, Geo- and Environmental Science
Gregor Schmid and Martin Obst.
Recenzii
From
the
book
reviews:
“The amount of information in this book is impressive. … The layout of the book is well-organized and the protocols with their accompanying notes are particularly useful and comprehensive. … I would highly recommend this book to any EM laboratory that does not already have the second edition and to larger EM research facilities that are performing the latest correlative microscopy and image analysis techniques.” (Kath White, Microscopy and Microanalysis, Vol. 20 (5), October, 2014)
“The amount of information in this book is impressive. … The layout of the book is well-organized and the protocols with their accompanying notes are particularly useful and comprehensive. … I would highly recommend this book to any EM laboratory that does not already have the second edition and to larger EM research facilities that are performing the latest correlative microscopy and image analysis techniques.” (Kath White, Microscopy and Microanalysis, Vol. 20 (5), October, 2014)
Textul de pe ultima copertă
This
third
edition
of
Electron
Microscopy:
Methods
and
Protocols
expands
upon
the
previous
editions
with
current,
detailed
protocols
on
biological
and
molecular
research
techniques
based
on
TEM
and
SEM
as
well
as
other
closely
related
imaging
and
analytical
methods.
With
new
chapters
on
conventional
and
microwave
assisted
specimen,
cryo-specimen
preparation,
negative
staining
and
immunogold
labelling
techniques,
DNA
and
RNA
tracking
using
hybrization
in
TEM
or
Atomic
Force
Microscopy,
TEM
crystallography
and
cryoTEM
3D
tomography,
3D
tomography
of
resin
embedded
tissues
using
FIB-SEM
,
Correlative
microscopy
using
fluorescence
microscopy,
confocal
microscopy
or
immune
labelling
techniques
for
both
TEM
and
FIB-SEM,
and
Elemental
and
isotopic
identification
and
their
distribution
in
cells
and
tissues
using
TEM,
SEM,
Scanning
Transmission
Electron
Microscopy
(STEM),
Secondary
Ion
Mass
Spectrometry
(SIMS)
and
NanoSIMS.
Written
in
the
highly
successful
Methods
in
Molecular
Biology
series
format,
chapters
include
introductions
to
their
respective
topics,
lists
of
the
necessary
materials
and
reagents,
step-by-step,
readily
reproducible
laboratory
protocols,
and
tips
on
troubleshooting
and
avoiding
known
pitfalls.
Authoritative and practical, Electron Microscopy: Methods and Protocols, Third Edition provides the most up-to-date and essential information in electron microscopy techniques and methods provided in this edition will assist in advancing future molecular and biological research.
Authoritative and practical, Electron Microscopy: Methods and Protocols, Third Edition provides the most up-to-date and essential information in electron microscopy techniques and methods provided in this edition will assist in advancing future molecular and biological research.
Caracteristici
Deals
with
biological
and
molecular
research
techniques
based
on
TEM
and
SEM
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts