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Dendritic Cell Protocols de Elodie Segura

Dendritic Cell Protocols: Methods in Molecular Biology, cartea 1423

Editat de Elodie Segura, Nobuyuki Onai
en Limba Engleză Paperback – 26 mai 2018
The third edition of thisvolume is aimed at providing both beginners and more experienced researchers achoice of methods to isolate and analyze dendritic cells(DC). An introductoryreview provides an overview of recent advances in the characterization of DCsubsets in mouse and human. While additional chapters provide methods toculture human and mouse dendritic cells, protocols for the isolation ofdendritic cells, the isolation of dendritic cell progenitors from mouse, andthe purification of dendritic cells from human blood. Written in the highlysuccessful Methods in Molecular Biology series format, chapters includeintroductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls.
 
Authoritative andcutting-edge,  Dendritic CellProtocols, Third Edition aims to ensure successful results in the furtherstudy of this vital field.
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Specificații

ISBN-13: 9781493980925
ISBN-10: 1493980920
Pagini: 322
Ilustrații: XI, 322 p. 68 illus., 27 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.59 kg
Ediția:Softcover reprint of the original 3rd ed. 2016
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Review of Mouse and Human Dendritic Cell Subsets.- InVitro Generation of Human Xcr1+ Dendritic Cells from Cd34+Hematopoietic Progenitors.- Derivationand Utilization Of Functional Cd8+ Dendritic Cell Lines.- Isolationof Dendritic Cell Progenitor and Bone Marrow Progenitor Cells from Mouse.-  TheIsolation and Enrichment of Large Numbers of Highly Purified Mouse SpleenDendritic Cell Populations and their InVitro Equivalents.- DendriticCell Subsets Purification from Human Tonsils and Lymph Nodes.- Isolationand Identification of Conventional Dendritic Cell Subsets from the Intestine ofMice and Men.- Isolationof Human Skin Dendritic Cell Subsets.- Isolation of Mouse Dendritic Cell Subsets andMacrophages from Skin.- Isolation of Conventional Dendritic Cells fromMouse Lungs.- Purificationof Human Dendritic Cell Subsets from Peripheral Blood.- Protocols for the Identification andIsolation ofAntigen-Presenting Cells in Human and Mouse Tissues.- Measurement of Export to the Cytosol in DendriticCells Using a Cytofluorimetry-Based Assay.- Cross-Presentation Assay for Human Dendritic Cells.- Analysis ofIntracellular Trafficking of Dendritic Cell Receptors for Antigen Targeting.- Characterizationof Dendritic Cell Subsets through Gene Expression Analysis.- In Vivo Ablation of a Dendritic Cell SubsetExpressing a Chemokine Receptor, Xcr1.- In Vivo Analysis Of IntestinalMononuclear Phagocytes.- In Vivo Imaging Of Cutaneous Dcs In Mice.- Analysis of Dendritic Cell Function by Clec9a-DtrTransgenic Mice.- Analysisof Dc Functions Using Cd205-Dtr Knock-In Mice.- Generation of Humanized Mice for Analysis ofHuman Dendritic Cells.



Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras