Designed for undergraduates, graduate students, and industry practitioners, the third edition of Bioseparations Science and Engineering fills a critical need in the field. Current, comprehensive, and concise, it covers bioseparations unit operations in unprecedented depth. The unit operations covered are cell lysis, flocculation, filtration, sedimentation, extraction, liquid chromatography, liquid adsorption, precipitation, crystallization, evaporation, and drying. In each of the chapters, the authors use a consistent method of explaining unit operations, starting with a qualitative description noting the significance and general application of the unit operation. They then illustrate the scientific application of the operation, develop the required mathematical theory, and finally, describe the applications of the theory in engineering practice, with an emphasis on design and scale-up. Unique to this text is a chapter dedicated to bioseparations process design and economics, in which a process simulator, SuperPro Designer®, is used to analyze and evaluate the production of six important biological products. The third edition of the book has been completely updated and contains the addition of several topics, including the stability of bioproducts, electrophoretic analysis of DNA and RNA, separation by flow cytometry, continuous crystallization, batch crystallization by cooling, fluidized bed drying, and process design and economics of the production of messenger RNA vaccine, hyaluronic acid, and monosodium glutamate. Unique features include basic information about bioproducts, descriptions of analytical methods and bench scale separations of bioproducts, and a chapter with bioseparations laboratory exercises. Bioseparations Science and Engineering is ideal for students and professionals working in or studying bioseparations and is the premier text in the field.