The Olfactory System: Methods in Molecular Biology

Visualizing and Manipulating Olfactory Cilia through Viral Delivery Coupled with En Face Imaging of Intact OE.- Genome-Wide RNA Tomography in the Mouse Whole Olfactory Mucosa.- Optical Activation of Photoswitchable TRPC Ligands in the Mammalian Olfactory System Using Laser Scanning Confocal Microscopy.- Intranasal Pressure Recording for Monitoring Mouse Respiration.- Identification of Immune Cells in Murine Olfactory Mucosa.- Chromatin Immunoprecipitation from Formaldehyde Cross-Linked Olfactory Sensory Neurons.- Activity-Dependent Labeling of Olfactory Sensory Neurons using RNA Fluorescence In Situ Hybridization followed by Phospho-S6 Immunofluorescence.- Measuring Cell Surface Expression of Odorant Receptors Via Flow Cytometry.- Dissociation of Mouse Olfactory Mucosae for Fluorescence-Activated Cell Sorting of Olfactory Sensory Neurons.- Preparation of Human Olfactory Epithelial Biopsies for Downstream Analysis.- Cranial Window for Acute and Chronic Optical Access to Record Neuronal Network Dynamics in the Olfactory Bulb.- Target-Captured mRNA from Murine Olfactory Bulb.- Identification and Localization of Cell Types in the Mouse Olfactory Bulb using Slide-SeqV2.- Dense and Sparse Labelling of Mitral Cells by Oral and Intraperitoneal Routes of Tamoxifen Administration.- Techniques in Staining of Rodent and Human Olfactory Tissue.- Electrophysiological Recordings from Identified Cell-Types in the Olfactory Cortex of Awake Mice.

Thorough and cutting-edge, The Olfactory System: Methods and Protocols is a valuable resource for all researchers who are interested in learning more about this important and developing field.