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T-Cell Development de Rémy Bosselut

T-Cell Development

Editat de Rémy Bosselut, Melanie S. Vacchio
en Limba Engleză Hardback – 22 aug 2015
​This volume provides simple and accessible experiment protocols to explore thymus biology. T-Cell Development: Methods and Protocols is divided into three parts presenting short reviews on T cell development, analysis strategies, protocols for cell preparation, flow cytometry analyses, and multiple aspects of thymocyte biology. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.  Concise and easy-to-use, T-Cell Development: Methods and Protocols aims to ensure successful results in the further study of this vital field.
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Specificații

ISBN-13: 9781493928088
ISBN-10: 1493928082
Pagini: 312
Ilustrații: XI, 297 p. 52 illus., 24 illus. in color.
Dimensiuni: 183 x 260 x 23 mm
Greutate: 0.79 kg
Ediția:1st edition 2015
Editura: Springer
Locul publicării:New York, NY, United States

Public țintă

Professional/practitioner

Cuprins

200 Million Thymocytes and I: A Beginner’s Survival Guide to T cell Development.- Development of gd T Cells, the Special Forces Soldiers of the Immune System.- Genetic Tools to Study T Cell Development.- Assessment of T cell Development by Flow Cytometry.- Flow Cytometry Analysis of Thymic Cells and their Subpopulations.- Identifying the Spatial Relationships of Thymic Stromal and Thymocyte Subsets by Immunofluorescence Analysis.- Purification of Thymocyte and T Cell Subsets.- Retroviral Transduction of T Cells and T Cell Precursors.- Bone Marrow and Fetal Liver Radiation Chimeras.- In vitro Analyses of T Cell Effector Differentiation.- Studying T Cell Development in Thymic Slices.- FTOC-based Analysis of Negative Selection.- Reconstituted Thymus Organ Culture.- Induction of T-cell Development in vitro by Delta-like (Dll)-Expressing Stromal Cells.- In vitro Analysis of Thymocyte Signaling.- Molecular Analysis of Mouse T Cell Receptor a and b Gene Rearrangements.- Intrathymic Injection.- Analysis of Cell Proliferation and |Homeostasis using EdU Labeling.- Characterization and Isolation of Human T Cell Progenitors.- Approaches to Study Human T Cell Development.- Humanized Mice to Study Human T Cell Development.- Using the Zebrafish Model to Study T cell Development.

Textul de pe ultima copertă

This volume provides simple and accessible experiment protocols to explore thymus biology. T-Cell Development: Methods and Protocols is divided into three parts presenting short reviews on T cell development, analysis strategies, protocols for cell preparation, flow cytometry analyses, and multiple aspects of thymocyte biology. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
 Concise and easy-to-use, T-Cell Development: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts