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RNA Remodeling Proteins de Marc Boudvillain

RNA Remodeling Proteins: Methods in Molecular Biology, cartea 3081

Editat de Marc Boudvillain
en Limba Engleză Hardback – 13 oct 2026
This volume provides readers with current methods to study RNA remodeling proteins. The methods, ranging from basic to complex, help the scientific community understand the role and fate of RNA species in cells, and their structures and interactions with other biomolecules. The book begins with two introductory chapters, followed by chapters where readers will find procedures to identify RNA remodeling proteins and their cofactors, physiological RNA targets and biological functions, and complex molecular mechanisms of action using purified components. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, RNA Remodeling Proteins: Methods and Protocols seeks to aid scientists in the further study of this ever evolving field of proteins and mechanisms.
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Specificații

ISBN-13: 9781071655702
ISBN-10: 1071655701
Pagini: 544
Dimensiuni: 183 x 260 x 35 mm
Greutate: 1.15 kg
Ediția:Third Edition 2027
Editura: Garland Science
Colecția Methods in Molecular Biology
Seria Methods in Molecular Biology

Cuprins

Key points to consider when studying RNA remodeling by proteins.- Predicting RNA and protein:RNA complexes with AlphaFold: possibilities, limitations, and practical guidelines.- Guidelines to study transcriptome remodeling by proteins using deep sequencing (RNA-seq).- Tools for experimental and computational genome-wide analysis of the function of DDX5 and DDX17 helicases in RNA processing.- A Workflow for Identifying Helicase-Dependent RNA Structural Changes in Transcriptional Termination: From DMS Sequencing Analysis to Reporter Assay-Based Validation.- Using cross-linking and immunoprecipitation (CLIP) to identify targets and specificity determinants of the YTHDC2 helicase.- Genome-wide mapping of R-loops in S. cerevisiae by H-CRAC.- Global identification of RNA helicase substrates from large nucleic acid sequence libraries using Helicase-SELEX.- Identification of host interaction partners of the Hepatitis E Virus Internal Ribosome Entry Site like-RNA using RNA-protein interaction detection assay and yeast three-hybrid assay.- Purification of C/D-box snoRNP and fibrillarin associated factors from Arabidopsis thaliana plants using GFP-tagged proteins.- In Vitro RNA–Protein Binding Assays to Identify RNA Helicases for U snRNA Nuclear Export.- Quantifying Label-Free Protein–RNA Binding Using Microscale Thermophoresis.- Measuring the affinity of the bacterial Rho helicase for RNA using a double membrane slot-blot filtration assay.- Unwinding Quadruplex Structures with RNA Helicases.- Insights into the activities of helicases from Thermococcales: the cases of the RNA helicase ASH-Ski2, the DNA helicase Hel308 and the RNA:DNA helicase Lhr2.- Mapping of RNA helicase dynamics by HDX-MS.- Native Liquid MALDI (NALIM) mass spectrometry analysis of RNA-binding protein complexes.- Using solution NMR to study the RNA-binding properties and molecular mechanisms of the RNA helicase DDX3X.- Using magnetic tweezers to unravel the mechanism of the G-quadruplex binding and unwinding activities of DHX36 helicase.- In vitro assay of bacterial RNA helicases liquid-liquid phase separation.- Probing the role of RNA helicases in modulating stress granules, RNA localization and P-body docking.- Fast analysis of RNA from transcription and Rho-dependent termination assays using automated capillary electrophoresis.- Assays to study SUMOylation of RNA helicase DHX9.- Analysis of Bacillus subtilis ribonuclease activity in vivo.- Assay of Bacillus subtilis ribonuclease activity in vitro.

Textul de pe ultima copertă

This volume provides readers with current methods to study RNA remodeling proteins. The methods, ranging from basic to complex, help the scientific community understand the role and fate of RNA species in cells, and their structures and interactions with other biomolecules. The book begins with two introductory chapters, followed by chapters where readers will find procedures to identify RNA remodeling proteins and their cofactors, physiological RNA targets and biological functions, and complex molecular mechanisms of action using purified components. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, RNA Remodeling Proteins: Methods and Protocols seeks to aid scientists in the further study of this ever evolving field of proteins and mechanisms.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproductible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras