Protocols in Biochemistry and Clinical Biochemistry
Autor Shweta Pandey, Shyamal K Goswami, Buddhi Prakash Jainen Limba Engleză Paperback – 16 noi 2020
- Includes full listings and discussion of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods and troubleshooting
- Features clear, step-by-step protocols and instructions with color diagrams and images
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Specificații
ISBN-13: 9780128220078
ISBN-10: 0128220074
Pagini: 138
Dimensiuni: 191 x 235 x 14 mm
Greutate: 0.29 kg
Editura: ELSEVIER SCIENCE
ISBN-10: 0128220074
Pagini: 138
Dimensiuni: 191 x 235 x 14 mm
Greutate: 0.29 kg
Editura: ELSEVIER SCIENCE
Public țintă
Active researchers in biochemistry, molecular biology, and cell biologyCuprins
Unit I: Buffer, solution and pH1. Preparation of normal and molar solution2. Buffer preparation3. Determination of pKa, pH
Unit II: Nucleic Acids1. Isolation and Purification of DNA2. Isolation and Purification of RNA3. Purification of Nucleic Acid4. Quantitative estimation of Nucleic acid5. Agarose gel electrophoresis for separation of nucleic acid
Unit III: Carbohydrate and Lipids1. Qualitative test for carbohydrate (Benedict, Molisch etc)2. Qualitative test for Lipids3. Thin liquid chromatography (TLC for separation and identification of carbohydrate and lipids
Unit III: Proteins1. Isolation of Protein2. Qualitative test of Protein (Biuret test, Xanthoproteic test, Ninhydrin test etc)3. Estimation of Protein (Bradford, Lowry etc)4. Paper Chromatography for separation and identification of amino acid5. Ion exchange chromatography for separation of amino acid6. Protein purification7. SDS PAGE analysis of proteins8. Western blotting for protein analysis9. Isoelectric pH determination
Unit IV: Enzymes1. Enzyme kinetics2. Study of the activity of salivary amylase and the effect of pH, temperature, salts on its activity3. Enzymatic activity of Trypsin and Lipase4. Enzyme purification5. Determination of Km, Vmax and Ki
Unit V: Clinical test1. Acid and Alkaline phosphatase activity2. SGPT and SGOT test3. Estimation of Urea, Uric acid and Creatinine4. Estimation of Glucose5. Estimation of Cholesterol6. Estimation of Vitamins7. Glucose tolerance test8. Estimation of Ca2+ and T4 in serum9. Pregnancy test by HCG hormone10. Estimation of Glycosylated haemoglobin
Unit II: Nucleic Acids1. Isolation and Purification of DNA2. Isolation and Purification of RNA3. Purification of Nucleic Acid4. Quantitative estimation of Nucleic acid5. Agarose gel electrophoresis for separation of nucleic acid
Unit III: Carbohydrate and Lipids1. Qualitative test for carbohydrate (Benedict, Molisch etc)2. Qualitative test for Lipids3. Thin liquid chromatography (TLC for separation and identification of carbohydrate and lipids
Unit III: Proteins1. Isolation of Protein2. Qualitative test of Protein (Biuret test, Xanthoproteic test, Ninhydrin test etc)3. Estimation of Protein (Bradford, Lowry etc)4. Paper Chromatography for separation and identification of amino acid5. Ion exchange chromatography for separation of amino acid6. Protein purification7. SDS PAGE analysis of proteins8. Western blotting for protein analysis9. Isoelectric pH determination
Unit IV: Enzymes1. Enzyme kinetics2. Study of the activity of salivary amylase and the effect of pH, temperature, salts on its activity3. Enzymatic activity of Trypsin and Lipase4. Enzyme purification5. Determination of Km, Vmax and Ki
Unit V: Clinical test1. Acid and Alkaline phosphatase activity2. SGPT and SGOT test3. Estimation of Urea, Uric acid and Creatinine4. Estimation of Glucose5. Estimation of Cholesterol6. Estimation of Vitamins7. Glucose tolerance test8. Estimation of Ca2+ and T4 in serum9. Pregnancy test by HCG hormone10. Estimation of Glycosylated haemoglobin
Recenzii
"...provides an encyclopedic description of about 100 different laboratory techniques for analyzing biomolecules including lipids, carbohydrates, proteins, enzymes, oligonucleotides, vitamins, and does so in a single, compact volume. The purpose of the book is to offer clear hands-on instruction on biochemistry methods and protocols. The book’s audience is the students, postdoctoral trainees, technicians, and lead scientists who work day-to-day at the benches of hospital and clinical labs; academic research laboratories; industrial research and development, quality assurance, and quality control labs; and environmental monitoring and waste treatment facilities.... [It] contains nearly double the content of the previous edition, which was published in 2000.... In this spectrum, this book occupies an important niche as a broadly applicable, yet compact and straightforward, resource that should be of great utility to scientists of all types who find themselves needing to analyze biologically derived or clinical materials,... it is highly recommended." ©Doody's Review Service, 2025, Peter Kennelly, PhD (Virginia Tech)