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Protein Dynamics: Methods and Protocols de Dennis R. Livesay

Protein Dynamics: Methods and Protocols: Methods in Molecular Biology, cartea 1084

Editat de Dennis R. Livesay
en Limba Engleză Hardback – 24 sep 2013
In Protein Dynamics: Methods and Protocols, expert researchers in the field detail both experimental and computational methods to interrogate molecular level fluctuations. Chapters detail best-practice recipes covering both experimental and computational techniques, reflecting modern protein research. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and practical, Protein Dynamics: Methods and Protocols describes the most common and powerful methods used to characterize protein dynamics.
 
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Specificații

ISBN-13: 9781627036573
ISBN-10: 1627036571
Pagini: 300
Ilustrații: XIV, 285 p. 82 illus., 56 illus. in color.
Dimensiuni: 178 x 254 x 22 mm
Greutate: 0.7 kg
Ediția:2014
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Public țintă

Professional/practitioner

Cuprins

Monitoring Side-Chain Dynamics of Proteins Using 2H Relaxation.- CPMG Relaxation Dispersion.- Confocal Single-Molecule FRET for Protein Conformational Dynamics.- Protein Structural Dynamics Revealed by Site-directed Spin Labeling and Multifrequency EPR.- Probing Backbone Dynamics With Hydrogen/Deuterium Exchange Mass Spectrometry.- Carbon-Deuterium Bonds as Non-perturbative Infrared Probes of Protein Dynamics, Electrostatics, Heterogeneity, and Folding.- Balancing Bond, Nonbond and Gō-like Terms in Coarse Grain Simulations of Conformational Dynamics.- Tutorial on Building Markov State Models with MSMBuilder and Coarse-graining them with BACE.- Analysis of Protein Conformational Transitions Using Elastic Network Model.- Geometric Simulation of Flexible Motion in Proteins.- Principal Component Analysis: A Method for Determining the Essential Dynamics of Proteins.- A Case Study Comparing Quantitative Stability/Flexibility Relationships Across Five Metallo-β-Lactamases Highlighting Differences within NDM-1.- Towards Comprehensive Analysis of Protein Family Quantitative Stability/Flexibility Relationships using Homology Models.- Using the COREX/BEST Server to Model the Native State Ensemble.- Morphing Methods to Visualize Coarse-grained Protein Dynamics.

Textul de pe ultima copertă

In Protein Dynamics: Methods and Protocols, expert researchers in the field detail both experimental and computational methods to interrogate molecular level fluctuations. Chapters detail best-practice recipes covering both experimental and computational techniques, reflecting modern protein research. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and practical, Protein Dynamics: Methods and Protocols describes the most common and powerful methods used to characterize protein dynamics.
 

Caracteristici

Details both experimental and computational methods to interrogate molecular level fluctuations Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts