Practical Protein Chromatography

Thiophilic Adsorption Chromatography.- Immobilized Metal Ion Affinity Chromatography.- Histidine Ligand Affinity Chromatography.- Boronic Acid Matrices for the Affinity Purification of Glycoproteins and Enzymes.- Calcium-Dependent Hydrophobic Interaction Chromatography.- Lectin Affinity Chromatography.- Dye-Ligand Affinity Chromatography.- High-Performance Affinity Chromatography for Protein Separation and Purification.- Immunoafflnity Chromatography.- Selection of Antibodies for Immunoaffinity Chromatography.- Some Alternative Coupling Chemistries for Affinity Chromatography.- Exploiting Weak Affinities.- Biospecific Affinity Elution.- Size-Exclusion High-Performance Liquid Chromatography of Proteins.- Chromatofocusing.- Ion-Exchange Chromatography of Proteins.- Displacement Chromatography of Proteins.- Purification of DNA Binding Proteins by Affinity and Ion Exchange Chromatography.- Making Bacterial Extracts Suitable for Chromatography.- Determination of Purity and Yield.

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