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PCR de Lucília Domingues

PCR

Editat de Lucília Domingues
en Limba Engleză Paperback – 23 aug 2023
This volume details PCR technique with focus on its application specificities to the biotechnology and bioengineering field.  Chapters are broken into five sections covering sgeneral PCR protocols, different applied examples to molecular and synthetic biotechnology, food science and technology, environmental microbiology and molecular ecology,  and healthcare. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, PCR: Methods and Protocols hopes to be a valuable and useful resource for wet lab researchers, particularly within the biotechnology and bioengineering field.
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Specificații

ISBN-13: 9781071633601
ISBN-10: 1071633600
Pagini: 268
Dimensiuni: 178 x 254 x 15 mm
Greutate: 0.51 kg
Ediția:Second Edition 2023
Editura: Humana

Cuprins

Digital PCR: A partitioning-based application for detection and surveillance of SARS-CoV-2 from sewage samples.- Digital PCR: A tool to authenticate herbal products and spice.- Emulsion Polymerase Chain Reaction coupled with Denaturant Gradient Gel Electrophoresis for microbial diversity studies.- Real-Time PCR High-Resolution Melting Assays for the Detection of Foodborne Pathogens.- High-Throughput real-time qPCR and High-Resolution Melting (HRM) assay for fungal detection in plant matrices.- Multiplex real-time PCR for the detection of Shiga Toxin-producing Escherichia coli in foods.- DNA isolation from cocoa-derived products and cocoa authentication by TaqMan real-time PCR.- Quantitative real-time PCR for the detection of allergenic species in foods.- Accurate absolute quantification of bacterial populations in mixed cultures by qPCR.- Real-time PCR method for assessment of ParA-mediated recombination efficiency in minicircle production.- Gene expression quantification from pathogenic bacterial biofilms by quantitative PCR.- A real-time quantitative PCR protocol for the quantification of plasmid copy number in Lactococcus lactis.- Improved PCR by the use of disruptors, a new class of oligonucleotide reagents.- Mitochondrial DNA D-loop amplification and sequencing for species differentiation in milk.- Long-range  Polymerase Chain Reaction.- Megaprimer-based PCR to synthesise fusion genes for cloning.- Bacteria and Yeast ColonyPCR.- Inverse PCR for site-directed mutagenesis.- Optimized design of degenerate primers for PCR based on DNA or protein sequence comparisons. 

 

Textul de pe ultima copertă

This volume details PCR technique with focus on its application specificities to the biotechnology and bioengineering field.  Chapters are broken into five sections covering sgeneral PCR protocols, different applied examples to molecular and synthetic biotechnology, food science and technology, environmental microbiology and molecular ecology,  and healthcare. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, PCR: Methods and Protocols hopes to be a valuable and useful resource for wet lab researchers, particularly within the biotechnology and bioengineering field.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras