Muscle Stem Cells
Editat de Eusebio Perdiguero, Ddw Cornelisonen Limba Engleză Hardback – 2 mar 2017
Authoritative and cutting-edge, Muscle Stem Cells: Methods and Protocols aims to ensure successful results in the further study of this vital field.
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Specificații
ISBN-13: 9781493967698
ISBN-10: 149396769X
Pagini: 380
Ilustrații: XII, 365 p. With online files/update.
Dimensiuni: 183 x 260 x 26 mm
Greutate: 0.91 kg
Ediția:1st edition 2017
Editura: Humana
Locul publicării:New York, NY, United States
ISBN-10: 149396769X
Pagini: 380
Ilustrații: XII, 365 p. With online files/update.
Dimensiuni: 183 x 260 x 26 mm
Greutate: 0.91 kg
Ediția:1st edition 2017
Editura: Humana
Locul publicării:New York, NY, United States
Cuprins
Muscle Stem Cells: A Model System for Adult Stem Cell Biology.- Isolation of Muscle Stem Cells From Mouse Skeletal Muscle.- Primary Mouse Myoblast Purification using Magnetic Cell Separation.- Isolation, Culture, and Immunostaining of Skeletal Muscle Myofibers from Wildtype and Nestin-GFP Mice as a Means to Analyze Satellite Cells.- Characterization of Drosophila Muscle Stem Cell-like Adult Muscle Precursors.- Using Transgenic Zebrafish to Study Muscle Stem/Progenitor Cells.- Muscle Interstitial Cells: A Brief Field Guide to Non-satellite Cell Populations in Skeletal Muscle.- Isolation and Characterization of Vessel-Associated Stem/Progenitor Cells from Skeletal Muscle.- Fibro/Adipogenic Progenitors (FAPs): Isolation by FACS and Culture.- Single Cell Gene Expression Profiling of Skeletal Muscle-Derived Cells.- Engraftment of FACS Isolated Muscle Stem Cells into Injured Skeletal Muscle.- Transplantation of Skeletal Muscle Stem Cells.- Simultaneous Measurement of Mitochondrial and Glycolytic Activity in Quiescent Muscle Stem Cells.- Monitoring Autophagy in Muscle Stem Cells.- Mimicking Muscle Stem Cell Quiescence in Culture: Methods for Synchronization in Reversible Arrest.- Methods for Observing and Quantifying Muscle Satellite Cell Motility and Invasion In Vitro.- Effects of Macrophage Conditioned-medium on Murine and Human Muscle Cells: Analysis of Proliferation, Differentiation, and Fusion.- Optimization of Satellite Cell Culture through Biomaterials.- Systematic Identification of Genes Regulating Muscle Stem Cell Self-Renewal and Differentiation.- Bioinformatics for Novel Large Intergenic Non-coding RNA (lincRNA) Identification in Skeletal Muscle Cells.
Caracteristici
Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras