Mouse Genetics (Methods in Molecular Biology) de Shree Ram Singh

Mouse Genetics: Methods and Protocols: Methods in Molecular Biology, cartea 1194

Shree Ram Singh

Vincenzo Coppola

Mouse Genetics: Methods and Protocols provide selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages and modeling human diseases. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, a list of necessary materials, systematic, readily reproducible methods and a notes section, which shares tips on troubleshooting in order to avoid known pitfalls.
Comprehensive and authoritative, Mouse Genetics: Methods and Protocols promises to deliver fundamental techniques and protocols to geneticists, molecular biologists, cell and developmental biologists, students and postdoctoral fellows working in the various disciplines of mouse biology and modeling human disease.

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Springer – 17 sep 2016	569^.19 lei 7-9 săpt.
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Springer – 26 iul 2014	768^.18 lei 7-9 săpt.

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Cuprins

Part I: Mouse Genetics Techniques

1. Development of Pronuclear Injection-Based Targeted Transgenesis in Mice Through Cre–loxP Site-Specific Recombination
            Masato Ohtsuka

2. Generation of Conditional Knockout Mice
            Kazuhito Sakamoto, Channabasavaiah B. Gurumurthy and Kay-Uwe Wagner

3. Generating Mouse Lines for Lineage Tracing and Knockout Studies
            Petra Kraus, V. Sivakamasundari, Xing Xing, and Thomas Lufkin

4. Generation of an Allelic Series of Knock-In Mice Using Recombinase-Mediated Cassette Exchange (RMCE)
            Anton J.M. Roebroek and Bart Van Gool

5. Generating Chimeric Mice from Embryonic Stem Cells via Vial Coculturing or Hypertonic Microinjection
            Kun-Hsiung Lee

6. Genetic Inducible Fate Mapping in Adult Mice Using Tamoxifen-Dependent Cre Recombinases
            Susanne Feil, Jana Krauss, Martin Thunemann, and Robert Feil

7. Intra cytoplasmic Sperm Injection (ICSI)-Mediated Transgenesis in Mice
            Pedro N. Moreira and Lluís Montoliu

8. Generation of Bacterial Artificial Chromosome (BAC) Transgenic Mice
            Jane Beil and Thorsten Buch

9. Generation of Genetically Engineered Mice by the piggyBac Transposon System
            Sheng Ding, Tian Xu, and Xiaohui Wu

10. Generation and Applications of MADM-Based Mouse Genetic Mosaic System
            Hui Zong

11. Generation of Mouse Lines Conditionally Over-Expressing MicroRNA Using the Rosa26-Lox-Stop-Lox System
            Claudia Piovan, Foued Amari, Francesca Lovat, Qun Chen, and Vincenzo Coppola

12. In Situ Hybridization (Both Radioactive and Non-Radioactive) and Spatiotemporal Gene Expression Analysis
            Olga Simmons, Esther M. Bolanis, Jian Wang, and Simon J. Conway

Part II: Mouse Genetics in Stem Cells

13. Isolation and Handling of Mouse Embryonic Fibroblasts
            Kanika Jain, Paul J. Verma, and Jun Liu

14. Generation of Induced Pluripotent Stem Cells from Mouse Adipose Tissue
            Pollyanna Agnes Goh and Paul J. Verma

15. Pdx1 (GFP/w) Mice for Isolation, Characterization, and Differentiation of Pancreatic Progenitor Cells
            Michael D. Williams, Wilson Wong, Amanda Rixon, Sarang N. Satoor, Anandwardhan A. Hardikar, and Mugdha V. Joglekar

16. Generation of Transgenic Mouse Fluorescent Reporter Lines for Studying Hematopoietic Development
            Andrei M. Vacaru, Joseph Vitale, Johnathan Nieves, and Margaret H. Baron

17. Analyzing Gene Function in Adult Long-Term Hematopoietic Stem Cells Using the Interferon Inducible Mx1-Cre Mouse System
            Kristbjorn Orri Gudmundsson, Kevin Oakley, Yufen Han, and Yang Du

18. Generation of Transgenic Mice by Exploiting Spermatogonial Stem Cells In Vivo
            Lalit Sehgal, Abul Usmani, Sorab N. Dalal, and Subeer S. Majumdar

Part III: Mouse Genetics in Modeling Human Disease

19. Methods for the Detection of Genome Instability Derived from Replication Stress in Primary Mouse Embryonic Fibroblasts
            Spencer W. Luebben, Naoko Shima, and Tsuyoshi Kawabata

20. Transgenic Nude Mice Ubiquitously-Expressing Fluorescent Proteins for Color-Coded Imaging of the Tumor Microenvironment
            Robert M. Hoffman

21. Genetically Engineered Insertional Mutagenesis in Mice to Model Cancer: Sleeping Beauty
            Viive M. Howell and Emily K. Colvin

22. Generating Double Knockout Mice to Model Genetic Intervention for Diabetic Cardiomyopathy in Humans
            Vishalakshi Chavali, Shyam Sundar Nandi, Shree Ram Singh, and Paras Kumar Mishra

23. Experimental Osteoarthritis Models in Mice
            Julia Lorenz and Susanne Grässel

24. Mouse Models and Methods for Studying Human Disease, Acute Kidney Injury (AKI)
            Ganesan Ramesh and Punithavathi Ranganathan

25. Mouse Models of Acute and Chronic Colitis
            Santhakumar Manicassamy and Indumathi Manoharan

26. Manipulation and Assessment of Gut Microbiome for Metabolic Studies
            Sarang N. Satoor, Deepak P. Patil, Holly Kristensen, Mugdha V. Joglekar, Yogesh Shouche, and Anandwardhan A. Hardikar

Textul de pe ultima copertă

Mouse Genetics: Methods and Protocols provides selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages, and modeling human diseases. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, a list of necessary materials, systematic, readily reproducible methods, and a notes section, which shares tips on troubleshooting in order to avoid known pitfalls.

Comprehensive and authoritative, Mouse Genetics: Methods and Protocols promises to deliver fundamental techniques and protocols to geneticists, molecular biologists, cell and developmental biologists, students, and postdoctoral fellows working in the various disciplines of mouse biology and modeling human disease.

Mouse Genetics: Methods and Protocols: Methods in Molecular Biology, cartea 1194

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Mouse Genetics: Methods and Protocols: Methods in Molecular Biology, cartea 1194

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