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Metagenomics: Methods and Protocols : Methods in Molecular Biology, cartea 2555

Editat de Wolfgang R. Streit, Rolf Daniel
en Limba Engleză Hardback – 29 oct 2022
This volume provides up-to-date and novel techniques for various screening technologies currently used in metagenomics and related areas. Starting with DNA/RNA isolation from environmental samples, the book continues by delving into areas such as current methods used to isolate DNA and construct metagenomic libraries, establishment of metagenome libraries in non-E. coli hosts, and topics like function-driven mining of metagenomic DNA, screening and analyzing protocols for a wide array of different genes encoding enzymes, bacterial viruses and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Metagenomics: Methods and Protocols, Third Edition provides a comprehensive collection of up-to-date metagenome protocols and tools for the recovery of many major types of biocatalysts and allows for the easy setup of these screens in microbiology laboratories.
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Specificații

ISBN-13: 9781071627945
ISBN-10: 1071627945
Ilustrații: XI, 287 p. 42 illus., 34 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.77 kg
Ediția:3rd ed. 2023
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

1. Construction of Small-Insert and Large-Insert Metagenomic Libraries
            Carola Simon and Rolf Daniel
 
2. Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a Universal cDNA as Universal Template for Marker Gene Studies
            Jacqueline Hollensteiner, Franziska Wemheuer, Dominik Schneider, Birgit Pfeiffer, and Bernd Wemheuer
 
3. Functional Metagenomics as a Tool to Tap into Natural Diversity of Valuable Biotechnological Compounds
            Nancy Weiland-Bräuer, Livia Saleh, and Ruth A. Schmitz
 
4. Functional and Sequence-Specific Screening Protocols for the Detection of Novel Antimicrobial Resistance Genes in Metagenomic DNA
            Supathep Tansirichaiya, William Hutton, and Adam P. Roberts  
5. Identification of PKS Gene Clusters from Metagenomic Libraries Using a Next Generation Sequencing Approach
            Alinne L.R. Santana-Pereira
 
6. Activity-Based Screening of Metagenomic Fosmid Libraries for Hydrogen-Uptake Enzymes
            Nicole Adam-Beyer and Mirjam Perner
 
7. Functional Metagenomics Approach for the Discovery of Novel Genes Encoding Phosphatase Activity
            Genis A. Castillo Villamizar, Heiko Nacke, and Rolf Daniel
 
8. Isolation of Genes Encoding Carbon Metabolism Pathways from Complex Microbial Communities
            Aranksha Thakor, Jiujun Cheng, and Trevor C. Charles
 
9. Screening Metagenomes for Algae Cell Wall Carbohydrates Degrading Hydrolases in Enrichment Cultures
            Jascha F.H. Macdonald, Ines Krohn, and Wolfgang R. Streit
 
10. The PET-Degrading Potential of Global Metagenomes: From In Silico Mining to Active Enzymes
            Jennifer Chow, Pablo Pérez-García, Robert F. Dierkes, Hongli Zhang, and Wolfgang R. Streit
 
11. High-Throughput Screening for Thermostable Polyester Hydrolases
            Yannick Branson, Christoffel P. S. Badenhorst, Lara Pfaff, Carolin Buchmann, Ren Wei, and Uwe T. Bornscheuer
 12. Metagenomic Screening of a Novel PET Esterase via In Vitro Expression System
            Yuchen Han, Robert F. Dierkes, and Wolfgang R. Streit
 
13. Assigning Functions of Unknown Enzymes by High Throughput Enzyme Characterization
            Patricia Molina, Laura Fernandez-Lopez, Peter N. Golyshin, and Manuel Ferrer
 
14. Isolation of a Host-Confined Phage Metagenome Allows the Detection of Phages Both Capable and Incapable of Plaque Formation
            Ines Friedrich and Robert Hertel
 
15. CRISPR-Cas9 Shaped Viral Metagenomes Associated with Bacillus subtilis
            Katharina Kohm, Veronika Theresa Lutz, Ines Friedrich, and Robert Hertel
 
16. Cloning and Expression of Metagenomic DNA in Streptomyces lividans and Its Subsequent Fermentation for Optimized Production
            Yuriy Rebets, Jan Kormanec, Andriy Lutzhetskyy, Kristel Bernaerts, and Jozef Anné
 
17. DNA-, RNA-, and Protein-Based Stable-Isotope Probing for High-Throughput Biomarker Analysis of Active Microorganisms
            Eleanor Jameson, Martin Taubert, Roey Angel, Sara Coyotzi, Yin Chen, Özge Eyice, Hendrik Schäfer, J. Colin Murrell, Josh D. Neufeld, and Marc G. Dumont

Textul de pe ultima copertă

This volume provides up-to-date and novel techniques for various screening technologies currently used in metagenomics and related areas. Starting with DNA/RNA isolation from environmental samples, the book continues by delving into areas such as current methods used to isolate DNA and construct metagenomic libraries, establishment of metagenome libraries in non-E. coli hosts, and topics like function-driven mining of metagenomic DNA, screening and analyzing protocols for a wide array of different genes encoding enzymes, bacterial viruses and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Metagenomics: Methods and Protocols, Third Edition provides a comprehensive collection of up-to-date metagenome protocols and tools for the recovery of many major types of biocatalysts and allows for the easy setup of these screens in microbiology laboratories.

Caracteristici

Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts

Descriere

Descriere de la o altă ediție sau format:
Metagenomics has proven to be a powerful tool for exploring the ecology, metabolic profiling, and comparison of complex microbial communities as well as its important applications in the mining of metagenomes for genes encoding novel biocatalysts and drug molecules for bioindustries. In Metagenomics: Methods and Protocols, expert researches provide an overview and introduction to basic methods commonly used in laboratories that have a strong background in microbial metagenomics. The book attempts to address all of the working steps involved in this crucial field, beginning with DNA isolation from soils and marine samples and continuing with the construction and screening of libraries, along with key advise involving bioinformatic tools available to analyze large metagenomic sequence data sets. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Metagenomics: Methods and Protocols serves as a very complete guide to available screening protocols for all major biocatalysts in order to allow for the easy setup of these screens in any microbiology lab.