Melanoma

Model Systems for the Study of Malignant Melanoma.- Generation of Functional Gene Knockout Melanoma Cell Lines by CRISPR-Cas9 Gene Editing.- Establishment and Characterization of Model Antigen-Expressing Melanoma Cell Lines.- A Fluorescence-Based Assay for Measuring Glucose Uptake in Living Melanoma Cells.- Indirect Co-Culture of Melanoma Cells with Stromal Cells Present in Their Microenvironment as a Method for Evaluation of Their Mutual Interactions.- A Flow Cytometric Assay for Investigating Melanoma Cell Adhesion to Lymphatic Endothelial Cells.- Wound Healing Assay for Melanoma Cell Migration.- Evaluating Melanoma Cell Migration/Invasion in Transwell Assays by Flow Cytometry.- Skin Organ Constructs for In Vitro Modeling of Melanoma Invasion.- Modeling Proliferative Versus Migratory Melanoma Cell Phenotypes Using Biofabricated Matrices.- Capture and Isolation of Circulating Melanoma Cells Using Photoacoustic Flowmetry.- A Rapid Exosome Isolation Using Ultrafiltration and Size Exclusion Chromatography (REIUS) Method for Exosome Isolation from Melanoma Cell Lines.- Post-Lymphadenectomy Analysis of Small Extracellular Vesicles from Lymphatic Drainage/Exudative Seroma of Melanoma Patients.- Imaging and Analysis of Melanoma-Secreted Extracellular Vesicles in Tumor-Draining Lymph Nodes.- Detection of Biomarkers for EMT-Associated Phenotype Switching in Melanoma-Derived Extracellular Vesicles.- Imaging and Isolation of Extravasation-Participating Endothelial and Melanoma Cells During Angiopellosis.- Quantitative Mapping of Organelle Positioning in Cultured Cells Using Semi-Automated Image Analysis Pipeline.- Design Strategies for Melanoma-Targeting Fluorescent Probes.- Multi-Analyte Responsive Fluorescent Sensing of Pathological Cellular States in Melanoma.- Radiopharmaceuticals for Melanoma Theranostics.- PET Imaging of Melanoma Using a Melanin-Targeted Probe.- SPECT Imaging of Melanoma Using a 99mTc-Labeled Melanin-Targeted Probe.- PET Imaging of Melanoma Using a Melanocortin 1 Receptor-Targeted Probe.- Preparation of Melanoma Tissue for Multiplex Immunofluorescence Histology Analysis of Immune Infiltration and Spatial Organization.- A Method for Quantifying Immune Infiltrates and Tertiary Lymphoid Structures in Murine Melanoma Tumors by Multiplex Immunofluorescence.- Methods for Analyzing Immune Infiltrates and Spatial Organization Within the Melanoma Microenvironment Using Multiplex Immunofluorescence.- Integrated Functional and Metabolomic Profiling of Melanoma Cells Treated with Redox-Active Compounds.- Serum Peptidomic Profiling for Biomarker Discovery in Canine Oral Melanoma and Squamous Cell Carcinoma via MALDI-TOF and LC-MS/MS.- Discovery of Salivary Metabolomic Biomarkers for Canine Oral Melanoma and Squamous Cell Carcinoma via CIL LC-MS Analysis.- Spatial Lipidomic Analysis Using Secondary Ion Mass Spectrometry (SIMS).- Unbiased Microbiome and Metabolomic Profiling of Fecal Samples from Patients with Melanoma.- Assessment of Cell-Free MicroRNA by NGS Whole Transcriptome Analysis in Cutaneous Melanoma Patients’ Blood.- High-Throughput Identification of miRNA–Target Interactions in Melanoma Using miR-CATCHv2.0.- Characterization of an Anti-Tryptophan 2,3-Dioxygenase Monoclonal Antibody and Use in a Melanoma Model.- Analytical Assessment of Fc-Receptor Binding of Therapeutic IgG1 Antibodies: A Surface Plasmon Resonance Approach.- In Vivo Reversible Electroporation for Melanoma Therapy.- Lipoplex Nanoparticle-Based RNA Delivery to 3D Melanoma Spheroids.- Assessing the Anti-Tumor Immune Effects of Gut Microbiota in a Preclinical Melanoma Model.- Generation of Murine Chimeric Antigen Receptor T Cells for Adoptive T Cell Therapy for Melanoma.- Retroviral Transduction of Human T Cells with a Chimeric Antigen Receptor Targeting PD 1 Ligands for Melanoma Immunotherapy.