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Lipases and Phospholipases: Methods in Molecular Biology, cartea 3064

Editat de Georgina Sandoval
en Limba Engleză Hardback – 24 sep 2026
Lipases are the most applied enzymes in organic synthesis due to their broad substrate acceptance and because of the availability of the molecular, biochemical, themodynamical and solvent engineering tools, which allows the optimization of lipases and lipase-catalyzed reactions. On the other hand, phospholipases are emerging as useful enzymes in food and pharmaceutical industries. In Lipases and Phospholipases: Methods and Protocols, expert researchers in the field provide key techniques to investigate these essential enzymes. Focusing on fundamental issues, current and new applications as well as practical step-by-step protocols, and the extensive applications of lipases and the potential application of phospholipases and its inhibitors. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.
 Thorough and intuitive, Lipases and Phospholipases: Methods and Protocols aids scientists in continuing to study lipases, phopholipases and related enzymes.
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Specificații

ISBN-13: 9781071654712
ISBN-10: 1071654713
Pagini: 352
Dimensiuni: 183 x 260 x 25 mm
Greutate: 0.83 kg
Ediția:Third Edition 2027
Editura: Humana
Colecția Methods in Molecular Biology
Seria Methods in Molecular Biology


Cuprins

Lipases: An Overview.- Phospholipases: Where Do We Stand?.- Quantitative Methods for Assessing True Lipase Activity Using Long-Chain Triglycerides and Complex Lipid Substrates.- Cloning and Protein Expression of CpEst in Komagataella phaffii.- Yarrowia lipolytica and Komagataella phaffii (Pichia pastoris) as Hosts for Heterologous Expression of Lipases.- Lipase Activity, Analytical Methods: Brief Review and Gold Standards.- Phospholipases D: An Update on Structure – Function Relationships.- Synthesis of Esters of 5-Hydroxymethylfurfural Catalyzed by Lipases in a Continuous Packed-Bed Bioreactor.- Synthesis and Purification of Lipase-Catalyzed Sugar E.- Lipase-Catalyzed Synthesis of Nutritional Structured Lipids at Laboratory-Scale Using Crude of Refined Oils.- Lipase-Catalyzed Pathways to the Synthesis of Pharmacologically Active Amides.- Lipases and Cutinases from Phytopathogenic and Saprobic Fungi in Plastic Depolymerization and Synthesis: A Review.- Biopolymers Derived from Solanum lycopersicum: Synthesis with CAL-B and Their Structural Analysis by Mass Spectrometry (DI-ESI and MALDI-TOF).- Functionalization of Flavonoids with Lipases.- Two-Step Lipase-Catalyzed Acylation of Non-Glycosylated Flavonoids: Application to Phloretin.- Assessment of the Hypoglycemic and Hypolipidemic Activities of Fructans and Their Laurate Bioconjugates.- Bio-Inspired Silica Lipase Nanobiocatalysts for the Synthesis of Fatty Acid Methyl Esters.- Identification of Domain Formation during sPLA2 Activity by Biophotonics.- Lipase/Esterase Activity as an Indicator of Milk Spoilage Process: A Sequential Injection Analysis Methodology.



Textul de pe ultima copertă

Lipases are the most applied enzymes in organic synthesis due to their broad substrate acceptance and because of the availability of the molecular, biochemical, themodynamical and solvent engineering tools, which allows the optimization of lipases and lipase-catalyzed reactions. On the other hand, phospholipases are emerging as useful enzymes in food and pharmaceutical industries. In Lipases and Phospholipases: Methods and Protocols, expert researchers in the field provide key techniques to investigate these essential enzymes. Focusing on fundamental issues, current and new applications as well as practical step-by-step protocols, and the extensive applications of lipases and the potential application of phospholipases and its inhibitors. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.
 Thorough and intuitive, Lipases and Phospholipases: Methods and Protocols aids scientists in continuing to study lipases, phopholipases and related enzymes.

Caracteristici

Includes cutting-edge methods and protocols for the study of lipases and phospholipases Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts