This book contains the refereed proceedings of the 16th International Conference on Knowledge Management in Organizations, KMO 2022, held in Hagen, Germany, in July 2022.  The 24 full papers and 5 short papers accepted for KMO 2022 were selected from 61 submissions and are organized in topical sections on: ​knowledge transfer and sharing; knowledge and organization; knowledge and service innovation; industry 4.0; information and knowledge systems; intelligent science; AI and new trends in KM.