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Imaging Mass Spectrometry : Methods and Protocols: Methods in Molecular Biology, cartea 1618

Editat de Laura M. Cole
en Limba Engleză Hardback – 19 mai 2017
This volume addresses the various techniques and novel applications of mass spectrometry imaging (MSI) and its role as a discovery tool in the field of proteomics, lipidomics, and metabolomics. The chapters in this book demonstrate how MSI can be applied to many areas of research such as clinical pathology, translational medicine, toxicology, biomarkers and response studies, and potential incorporation of MSI into forensic workflows. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Innovative and comprehensive, Imaging Mass Spectrometry: Methods and Protocols is a valuable resource for research scientists and clinicians who are interested in further studies of MSI technologies.
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Specificații

ISBN-13: 9781493970506
ISBN-10: 149397050X
Pagini: 204
Ilustrații: X, 204 p. 84 illus., 68 illus. in color.
Dimensiuni: 178 x 254 x 14 mm
Greutate: 0.59 kg
Ediția:1st ed. 2017
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Preface…
Table of Contents…
Contributing Authors…


1. ‘A Future Amalgamation between the Scientist and the Clinician?’
Sarah Haywood-Small


2. Fresh Frozen Versus Formalin Fixed Paraffin Embedded for Mass Spectrometry Imaging
Ekta Patel


3. Imaging MS of Rodent Ocular Tissues and the Optic Nerve
David M.G. Anderson, Wendi Lambert, David J. Calkins, Szolt Abloczy, Rosalie K. Crouch, Richard M. Caprioli, and Kevin L. Schey


4. MALDI-MSI of Lipids in Human Skin
Philippa J. Hart and Malcolm R. Clench
 
5. MALDI-MSI Analysis of Cytological Smears: The Study of Thyroid Cancer
Niccolò Mosele, Andrew Smith, Manuel Galli, Fabio Pagni, and Fulvio Magni


6. Droplet-Based Liquid Extraction for Spatially-Resolved Microproteomics Analysis of Tissue Sections
Maxence Wisztorski, Jusal Quanico, Julien Franck, Benoit Fatou, Michel Salzet, and Isabelle Fournier

7. DESI Mass Spectrometry Imaging (MSI)
Emmanuelle Claude, Emrys Jones, and Steven Pringle

8. Peptide Imaging: Maximizing Peptide Yield, Optimization of the ‘Peptide Mass Fingerprint’
Ekta Patel

9. MALDI-MS Imaging in the Study of Glomerulonephritis
Andrew Smith, Manuel Galli, Vincenzo L’Imperio, Fabio Pagni, and Fulvio Magni

10. Hierarchical Cluster Analysis to Aid Diagnostic Image Data Visualization of MS and Other Medical Imaging Modalities
Arul N. Selvan, Laura M. Cole, Lynne Spackman, Sarah Naylor, and Chris Wright

11. Laser Ablation Inductively Coupled Plasma Mass Spectrometry Imaging of Plant Metabolites
Callie Seaman

12. Mass Spectrometry Imaging of Drugs of Abuse in Hair
Bryn Flinders, Eva Cuypers, Tiffany Porta, Emmanuel Varesio, Gérard Hopfgartner, and Ron M.A. Heeren

13. MALDI Mass Spectrometry Profiling and Imaging Applied to the Analysis of Latent Fingermarks
R. Bradshaw

14.ToF-SIMS Parallel Imaging MS/MS of Lipid Species in Thin Tissue Sections
Anne L. Bruinen

15. Rodent Whole-Body Sectioning and MALDI Mass Spectrometry Imaging
Paul J. Trim

16. The Future in Disease Models for Mass Spectrometry Imaging, Ethical Issues and the Way Forward
Rebecca E. Day and Ieva Palubeckaite
 

Textul de pe ultima copertă

This volume addresses the various techniques and novel applications of mass spectrometry imaging (MSI) and its role as a discovery tool in the field of proteomics, lipidomics, and metabolomics. The chapters in this book demonstrate how MSI can be applied to many areas of research such as clinical pathology, translational medicine, toxicology, biomarkers and response studies, and potential incorporation of MSI into forensic workflows. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Innovative and comprehensive, Imaging Mass Spectrometry: Methods and Protocols is a valuable resource for research scientists and clinicians who are interested in further studies of MSI technologies.

Caracteristici

Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras