Host-Pathogen Interactions
Editat de Carlos Medina, Francisco Javier López-Baenaen Limba Engleză Paperback – 26 ian 2025
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Specificații
ISBN-13: 9781071636190
ISBN-10: 1071636197
Pagini: 284
Dimensiuni: 178 x 254 x 16 mm
Greutate: 0.54 kg
Ediția:Second Edition 2024
Editura: Humana
ISBN-10: 1071636197
Pagini: 284
Dimensiuni: 178 x 254 x 16 mm
Greutate: 0.54 kg
Ediția:Second Edition 2024
Editura: Humana
Cuprins
Genetic Association Studies in Host-Pathogens Interaction Analysis.- Bacterial Genotyping Methods: From the Basics to Modern.- Real-Time Reverse Transcription PCR as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens.- The Usage of a Bioluminescence Reporter System to Image Promoter Activity During Host Infection.- lacZ Reporter System as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens.- Western Blotting Against Tagged Virulence Determinants to Study Bacterial Pathogenicity.- Molecular Methods to Analyze the Effect of Proteins Expressed by Salmonella During Its Intracellular Stage.- Organoids as a Model to Study Infectious Disease.- Surface Proteome Biotinylation Combined with Bioinformatic Tools as a Strategy for Predicting Pathogen Interacting Proteins.- Systems Biology Modeling to Study Pathogen-Host Interactions.- Phage Therapy: Various Perspectives on How to Improve the Art.- Application of RNA-seq and Bioimaging Methods to Study Microbe-Microbe Interactions and Their Effects on Biofilm Formation and Gene Expression.- Serial Dilution-Based Growth Curves and Growth Curve Synchronization for High-Resolution Time Series of Bacterial Biofilm Growth.- Detection of Bacterial Quorum Sensing Molecules.- Generating Chromosome-Located Transcriptional Fusions to Fluorescent Proteins for Single-Cell Gene Expression Analysis in Pseudomonas syringae.- Introduction of Genetic Material in Ralstonia solanacearum through Natural Transformation and Conjugation.- In Vitro and In Vivo Secretion/Translocation Assays to Identify Novel Ralstonia solanacearum Type 3 Effectors.- Plant Pathogenicity Phenotyping of Ralstonia solanacearum Strains.- Methods to Quantify Biotic-Induced Stress in Plants.- From Sample to Data: Preparing, Obtaining, and Analyzing Images of Plant-Pathogen Interactions Using Confocal Microscopy.- Screening of c-di-GMP-Regulated Exopolysaccharides in Host Interacting Bacteria.- Primary Characterization of Small RNAs in Symbiotic Nitrogen-Fixing Bacteria.- A New, Non-Destructive, Split-Root System for Local and Systemic Plant Responses Studies with Soybean.- Methods for the Characterization of Plant-Growth Promoting Rhizobacteria.
Textul de pe ultima copertă
This fully updated volume serves as multidisciplinary compendium of approaches and techniques employed to analyze the role of different molecules, processes, or strategies used by different guests to survive and proliferate in their associations with eukaryotic hosts. Beginning with animal-pathogen interactions, the book then continues with chapters exploring virus-host interactions, plant-microbe interactions, as well as different molecular techniques that were initially applied to non-pathogenic interactions but can be adapted to study other host-pathogen associations. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Host-Pathogen Interactions: Methods and Protocols, Second Edition contributes to the study of host-pathogen interactions with numerous techniques that can be used in a variety of bacteria.
Caracteristici
Includes cutting-edge techniques for the study of host-pathogen interactions in animals and plants Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts