Cantitate/Preț
Produs
Total produse
Vezi coșul
Gene Transfer and Expression de Michael Kriegler

Gene Transfer and Expression

Autor Michael Kriegler
en Limba Engleză Paperback – 1990
A practical manual of protocols for achieving expression of foreign genes in mammalian cells. It includes some very new techniques such as PCR-based expression. The author gives a theoretical introduction to the protocols and compares the strengths and weaknesses.
Citește tot Restrânge

Toate formatele și edițiile

Toate formatele și edițiile Preț Express
Paperback (1) 38046 lei  6-8 săpt.
  Palgrave Macmillan UK – 1990 38046 lei  6-8 săpt.
Spirală (1) 24791 lei  45-50 zile
  Oxford University Press – 2 sep 1993 24791 lei  45-50 zile

Preț: 38046 lei

Nou

Puncte Express: 571
Preț estimativ în valută:
6733€ • 7897$ • 5904£

Carte tipărită la comandă

Livrare economică 26 ianuarie-09 februarie 26

 Adaugă în coș

Wish list
Gift list
Am citit!
Adaugă în listă
Preluare comenzi: 021 569.72.76

Specificații

ISBN-13: 9781349118939
ISBN-10: 1349118931
Pagini: 242
Ilustrații: XIII, 242 p.
Dimensiuni: 210 x 279 x 14 mm
Greutate: 0.59 kg
Ediția:1990 edition
Editura: Palgrave Macmillan UK
Locul publicării:London, United Kingdom

Cuprins

Part 1 Overview: general comparison of vectors and DNA transfer procedures; transient and stable expression systems; viral compared to DNA mediated transfer; stratefic overview - matching experimental procedures with goals and probability of success. Part 2 Vectors (DNA): vector components - organization of the most efficient vectors such as pMT2, pCD, SRa, EBV based, BPV based and T7 based vectors; control elements; retroviral vectors and recombinant retroviruses; PCR based expression; homologous recombination and gene replacement. Part 3 Cell lines and maintenance: general tissue culture techniques; growth conditions of most commonly used mammalian cells - COS, 3t3, CV1, CHO, C127, BHK, mouse L cells, Att20, MDCK, lymphoid cells; embryonal stem cells. Part 4 DNA transfer: calcium phosphate; DEAE dextran; protoplast fusion; electroporation; chromosome mediated transfer - DEPC/PEF fusion, gamma irradiation/PEG fusion; selecting transfer technique for cell type and stability of expression. Part 5 Selection and amplification methods: DHER; Ada; Neo; GPT; Hygro; ODC, Asn synthtase, mdr; negative selection protocols (gancyclovir). Part 6 Expression cloning: library construction; transfaction techniques; SIB selection. Part 7 Analysis of nucleic acid: isolation of RNA and DNA; analysis of RNA (traditional and PCR based); analysis of DNA (traditional and PCR based). Part 8 Analysis of protein: radiolabeling, immunoprecipitation and PAGE systems; western immunoblotting; analysis of glycosylation, sulfation and acylation; study of protein processing and secretion. Appendix: reagents - where to obtain and how to prepare; troubleshooting.

Descriere

Descriere de la o altă ediție sau format:
This book is a state-of-the-art catalogue of viral and cis-acting elements and the latest expression vectors for obtaining optimum expression of foreign genes introduced into mammalian cells. It describes in detail the essential techniques for propagating cells and cell lines, DNA transfer, drug selection and gene amplification, expression cloning, subtractive hybridizaton, retro-virus mediated gene transfer.