This book constitutes the refereed proceedings of the 12th International Conference on Functional Imaging and Modeling of the Heart, held in Lyon, France, in June 2023. The 72 full papers were carefully reviewed and selected from 80 submissions. The focus of the papers is on following topics: increased imaging resolutions, data explosion, sophistication of computational models and advent of AI frameworks, while new imaging modalities have emerged (e.g. combined PET-MRI, Spectral CT).