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Eosinophils: Methods and Protocols de Garry M. Walsh

Eosinophils: Methods and Protocols: Methods in Molecular Biology, cartea 1178

Editat de Garry M. Walsh
en Limba Engleză Hardback – 3 iul 2014
In Eosinophil: Methods and Protocols, experts in the field of eosinophil biology comprehensively provide detailed methodological insight into the study of this fascinating cell. This book is aimed at a diverse range of basic and clinical scientists who wish to work with eosinophils or who require an update of their knowledge or to gain the information required to study a function of the eosinophil different to their current area of enquiry.​ Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Eosinophil: Methods and Protocols seeks to aid scientist in the discovery of new hypotheses and for further examination of this intriguing cell.
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Specificații

ISBN-13: 9781493910151
ISBN-10: 1493910159
Pagini: 336
Ilustrații: XII, 324 p. 64 illus., 39 illus. in color.
Dimensiuni: 178 x 254 x 25 mm
Greutate: 0.8 kg
Ediția:2014
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Public țintă

Professional/practitioner

Cuprins

Eosinophil Overview - Structure, Biological Properties And Key Functions.- Eosinophil Purification From Peripheral Blood.- Eosinophil Purification From Human Bone Marrow.- CD34(+) Eosinophil-Lineage-Committed Cells In The Mouse Lung.- Eosinophil Cell Lines.- Cell Signalling In Eosinophil During Human Eosinophil Differentiation.- Eosinophil Intracellular Signalling – Apoptosis.- Identification Of Human Eosinophils In Whole Blood By Flow Cytometry.- Isolation And Functional Assessment Of Eosinophil Crystalloid Granules.- Eosinophil Chemotaxis.- Human Eosinophil Shape Change And Secretion.- Human Eosinophil Adhesion And Receptor Expression.- Adhesion Of Eosinophils To Endothelial Cells Or Substrates Under Flow Conditions.- Human Eosinophil Transmigration.- Measurement Of Eosinophil Kinetics In Healthy Volunteers.- Assays Of Eosinophil Apoptosis And Phagocytic Uptake.- Clinical Measurement Of Eosinophil Numbers In Eosinophilic Conjunctivitis.- Qualitative And Quantitative Studies Of Eosinophils In Parasitic Infections.- Interactions Of Eosinophils With Nerves.- Eosinophils Interaction With Mast Cells: The Allergic Effector Unit.- Eosinophil Interactions: Antigen Presentation.- Eosinophils And Respiratory Virus Infection: A Dual-Standard Curve Qrt-PCR-Based Method For Determining Virus Recovery From Mouse Lung Tissue.- Antimicrobial Activity Of Human Eosinophil Granule Proteins.- Eosinophils And The Ovalbumin Mouse Model Of Asthma.- Mutant Mice And Animal Models Of Allergic Disease.- Murine Models Of Eosinophilic Leukemia - A Model Of FIP1L1-PDGFRa Initiated Chronic Eosinophilic Leukemia/Systemic Mastocytosis.

Textul de pe ultima copertă

In Eosinophils: Methods and Protocols, experts in the field of eosinophil biology comprehensively provide detailed methodological insight into the study of this fascinating cell. This book is aimed at a diverse range of basic and clinical scientists who wish to work with eosinophils or who require an update of their knowledge or to gain the information required to study a function of the eosinophil different to their current area of enquiry.​Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and practical, Eosinophils: Methods and Protocols seeks to aid scientist in the discovery of new hypotheses and  for further examination of this intriguing cell.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Descriere

Descriere de la o altă ediție sau format:
The eosinophil is an enigmatic cell with a continuing ability to fascinate. In this book, experts in the field of eosinophil biology comprehensively update our knowledge on the human eosinophil in health and disease. Topics discussed include a synopsis of eosinophil characteristics, properties and role in disease. Important information on how eosinophils release their potent and toxic granule proteins will be covered and how these basic proteins give rise to pathologies including issues such as the function of the nerves.