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Confocal Microscopy: Methods and Protocols: Methods in Molecular Biology, cartea 1075

Editat de Stephen W. Paddock
en Limba Engleză Hardback – 20 sep 2013
Confocal Microscopy: Methods and Protocols, Second Edition takes the researcher from the bench top through the imaging process, to the page. Protocols for the preparation of tissues from many model organisms including worms, flies and mice have been included as well as chapters on confocal imaging of living cells, three dimensional analysis, and the measurement and presentation of confocal images for publication. Emphasis has been placed on the laser scanning confocal microscope since this is still the instrument used for most routine applications. The current generation of modern confocal instruments produces optical sections of cells and tissues that are free of out-of-focus fluorescence with reduced chances of artifacts from the techniques of specimen preparation. This allows the imaging of living specimens and measurements of physiological events within cells. Confocal microscopy has become essential in many fields of contemporary biomedical research where a light microscope is required for imaging fluorescently labeled cells and tissues, especially cell biology, developmental biology, neurobiology, and pathology. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
 
Authoritative and easily accessible, Confocal Microscopy: Methods and Protocols, Second Edition is aimed primarily, but not exclusively, at the novice user with pointers to more advanced techniques. 
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Specificații

ISBN-13: 9781588293510
ISBN-10: 1588293513
Pagini: 410
Ilustrații: XI, 381 p. 130 illus., 51 illus. in color.
Dimensiuni: 178 x 254 x 27 mm
Greutate: 0.86 kg
Ediția:2nd ed. 2014
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Public țintă

Professional/practitioner

Cuprins

1. Reflecting on Confocal Microscopy: A Personal Perspective
            John White
 
2.  Laser Scanning Confocal Microscopy: History, Applications And Related Optical Sectioning Techniques
            Stephen W. Paddock and Kevin W. Eliceiri
 
3. Confocal Microscopy on the Internet
            Kristin L. Hazelwood, Christopher S. Murphy, Zachary C. Cunningham, Matthew J. Parry-Hill, Richard L. Ludlow, Ericka B. Ramko, Anna P. Ozarowska, Adam M. Rainey and Michael W. Davidson
 
4. Using Photoshop with Images Created by a Confocal System
            Jerry Sedgewick
 
5. Clearing up the Signal: Spectral Imaging and Linear Unmixing in Fluorescence Microscopy
            Timo Zimmermann, Joanne Marrison, Karen Hogg, and Peter O’Toole
 
6. Low Magnification Confocal Microscopy of Tumor Angiogenesis
            George McNamara, Anna Yanai, Vazgen Khankaldyyan, Walter E. Laug, Jeff Boden, Keith Webster, Yiwen Li, and Rong Wen
 
7. Confocal Imaging of Butterfly Tissue
            Craig R. Brunetti
 
8. Confocal Microscopy of Cardiac Myocytes
            Robert L. Price, Stephen T. Haley, Tara Bullard, Jeffrey Davis, Thomas K. Borg, and Louis Terracio
 
9. Confocal Imaging of Fluorescently Labeled Proteins in the Drosophila Larval Neuromuscular Junction
            Ian P. Coyle
 
10. Confocal Imaging and Three-Dimensional Visualization of Thick Autofluorescent Specimens
            Angela V. Klaus, Valerie Schawaroch, and Kevin J. Frischmann
 
11. Vital Imaging of Multicellular Spheroids
            Paulo J. Oliveira, Ed Perkins, and Jon Holy
 
12. Live Confocal Analysis of Mutant and Drug Treated Drosophila Embryos
            Barbara Fasulo and William Sullivan
 
13. Confocal Imaging of the Microtubule Cytoskeleton in C. elegans Embryos and Germ Cells
            Kevin F. O’Connell and Andy Golden
 
14. Measurement in the Confocal Microscope
            Guy Cox
 
15. A Method for Quantifying Blood Flow Distribution Among The Alveoli of the Lung
            Robert L. Conhaim, Dennis M. Heisey, and Greg E. Leverson
 
16. Imaging Tools for Analysis of the Ureteric Tree in the Developing Mouse Kidney
            Luise A. Cullen-McEwen, Richard J. Young, Gabriel Fricout, Dominique Jeulin, Ian S. Harper, Frank Costantini, and John F. Bertram
 
17. Evaluating Confocal Microscopy System Performance
            Robert M. Zucker

Textul de pe ultima copertă

Confocal Microscopy: Methods and Protocols, Second Edition takes the researcher from the bench top through the imaging process, to the page. Protocols for the preparation of tissues from many model organisms including worms, flies and mice have been included as well as chapters on confocal imaging of living cells, three dimensional analysis, and the measurement and presentation of confocal images for publication. Emphasis has been placed on the laser scanning confocal microscope since this is still the instrument used for most routine applications. The current generation of modern confocal instruments produces optical sections of cells and tissues that are free of out-of-focus fluorescence with reduced chances of artifacts from the techniques of specimen preparation. This allows the imaging of living specimens and measurements of physiological events within cells. Confocal microscopy has become essential in many fields of contemporary biomedical research where a light microscope is required for imaging fluorescently labeled cells and tissues, especially cell biology, developmental biology, neurobiology, and pathology. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
 
Authoritative and easily accessible, Confocal Microscopy: Methods and Protocols, Second Edition is aimed primarily, but not exclusively, at the novice user with pointers to more advanced techniques. 

Caracteristici

Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras

Recenzii

"This book is an essential reference for anyone who is involved in the production of laser scanning confocal (LSCM) images. . . Exceptionally useful information on a great variety of methodologies that can be employed with LSCM is provided. . . In consideration of the vast quantity of useful information on all aspects of LSCM that is presented in this volume, and the fact that it is the first comprehensive book on this topic, it is highly recommended."-Quarterly Review of Biology

"...almost every research institute has a confocal microscope, and many are developing much larger multi-user biomedical imaging facilities. The publication of this collection of methods and protocols for confocal microscopy is therefore very timely...The tips and tricks used on the various systems should prove useful to a diverse range of confocal microscope users and would be a beneficial reference volume for any imaging unit and reasonable value for money."-Cell Biology International

" This is an excellent source for anyone who wants to explore techniques in confocal microscopy. It is a practical manual with many gems that will help both new and experienced users. The principles are efficiently and accessibly explained and there is a useful chapter on fluorescent probes...there is a wealth of ideas here waiting to be applied to prokaryotic biology and many would be equally useful for simple fluorescence rather than confocal studies. I would certainly recommend it to any lab that uses fluorescence microscopy extensively."-Microbiology Today

"...The texts are aimed at the biological user...for the user for whom they are intended, these essays contain a wealth of down-to-earth practical information. The book is well-produced and contains colour illustrations..."-Ultramicroscopy

"...excellent information as an introductory basis for confocal microscopy."-Cellular and Molecular Biology