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Chromosome Architecture: Methods and Protocols de Mark C. Leake

Chromosome Architecture: Methods and Protocols: Methods in Molecular Biology, cartea 1431

Editat de Mark C. Leake
en Limba Engleză Paperback – 26 mai 2018
This volume details a valuable collection of protocols andreviews, such as emerging experimental and theoretical approaches. Theseapproaches have resulted in a substantial improvement in the understanding ofchromosome architecture. Chromosome Architecture:Methods and Protocols guides readers through cutting-edge interdisciplinarymethods which allow for an understanding of architecture of chromosomes withexceptionally enhanced resolution, both in terms of space and time. Written in the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.
Authoritativeand cutting-edge, ChromosomeArchitecture: Methods and Protocols aims to ensure successful results in thefurther study of this vital field.

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Specificații

ISBN-13: 9781493981007
ISBN-10: 1493981005
Pagini: 290
Ilustrații: X, 290 p. 77 illus., 65 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.53 kg
Ediția:Softcover reprint of the original 1st ed. 2016
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

New Advances in Chromosome Architecture.- Single MoleculeNarrowfield Microscopy Of Protein-DNA Binding Dynamics In Glucose SignalTransduction Of Live Yeast Cells.- Single-Molecule Imaging to Characterise the TransportMechanism of the Nuclear Pore Complex.- Using Fluorescence Recovery after Photobleaching (FRAP)To Study Dynamics Of The Structural Maintenance Of Chromosome (SMC) Complex InVivo.- Imaging DNA Structure by Atomic Force Microscopy.- Investigating Bacterial ChromosomeArchitecture Using Epifluorescence Microscopy.- Transverse Magnetic TweezersAllowing Coincident Epi-Fluorescence Microscopy On Horizontally Extended DNA.- Probing Chromosome Dynamics in Bacillus Subtilis.- Systems Biology Approaches for Understanding Genome Architecture.- InVivo And In Situ Replication Labeling Methods For Super-ResolutionStructured Illumination Microscopy Of Chromosome Territories And ChromatinDomains.- DNA-Protein InteractionsStudied Directly Using Single Molecule Fluorescence Imaging Of Quantum DotTagged Proteins Moving On DNA Tightropes.- Escherichia Coli Chromosome CopyNumber Measurement Using Flow Cytometry Analysis.- Bacterial Chromosome Dynamics by Locus Tracking In FluorescenceMicroscopy.- Biophysical Characterization of Chromatin Remodelling Protein CHD4.- Atomistic MolecularDynamics Simulations of DNA Minicircle Topoisomers: A Practical Guide to Setup,Performance and Analysis.- Super-Resolution Microscopy and Tracking of DNA-Binding Proteins in Bacterial Cells.- Studying the Dynamics of Chromatin Binding Proteins in Mammalian CellsUsing Single-Molecule Localization Microscopy.- IntraNuclear Single Particle Tracking (I-SPT) To Reveal the Functional Architecture OfChromosomes.- Visualizing Bacillus Subtilis during Vegetative Growth and Spore Formation. 

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Textul de pe ultima copertă

This detailed new edition collects cutting-edge laboratory protocols, techniques, and applications in use by some of the leading international experts in the broad field of chromosome architecture. The book emphasizes the increasing physiological relevance of chromosome architecture investigation, manifest both through application of more complex bottom-up assays in vitro as well as through maintaining the native physiological context through the investigation of living, functional cells. In addition, the chapters reflect the dramatic improvement in the length scale of precision by utilizing single-molecule approaches, both for imaging the DNA content of chromosome and proteins that bind to DNA as well as using methods that can controllably manipulate single DNA molecules, and the use of advanced computational methods and mathematical analysis is also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Chromosome Architecture: Methods and Protocols, Second Edition is an ideal guide for researchers working in this dynamic area of study.