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Bioluminescent Imaging: Methods and Protocols de Steven Ripp

Bioluminescent Imaging: Methods and Protocols: Methods in Molecular Biology, cartea 2081

Editat de Steven Ripp
en Limba Engleză Hardback – 13 noi 2019
This volume provides detailed protocols and methodologies required to perform bioluminescent imaging in multiple stages, enabling the reader to integrate this technology into their laboratory-based imaging experiments. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 Authoritative and cutting-edge, Bioluminescent Imaging: Methods and Protocols to ensure successful results in the further study of this vital field.
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Specificații

ISBN-13: 9781493999392
ISBN-10: 1493999397
Pagini: 246
Ilustrații: XI, 246 p. 84 illus., 63 illus. in color.
Dimensiuni: 178 x 254 mm
Ediția:1st ed. 2020
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

High-throughput Bioluminescence Imaging and Reporter Gene Assay with 3D Spheroids from Human Cell Lines.- Bioluminescence Resonance Energy Transfer (BRET) Coupled Near-Infrared Imaging of Apoptotic Cells.- High-throughput Analysis of Endocrine Disrupting Compounds using BLYES and BLYAS Bioluminescent Yeast Bioassays.- Bioluminescent Imaging of Single Bacterial Cells Using an Enhanced ilux Operon.- Bioluminescent Imaging and Tracking of Bacterial Transport in Soils.- In vivo Bioluminescent Imaging of Yersinia ruckeri Pathogenesis in Fish.- In Vivo Bioluminescent Monitoring of Parasites in BALB/c Mouse Models of Cutaneous Leishmaniasis Drug Discovery.- Multiplex Imaging of Polymicrobial Communities – Murine Models to Study Oral Microbiome Interactions.- Bioluminescence Imaging to Study Mature Biofilm Formation by Candida spp. and Antifungal Activity in vitro and in vivo.- Bioluminescence Imaging of Neuroinflammation in a Mouse Model of Parkinson’s Disease.- Continual Conscious Bioluminescent Imaging in Freely Moving Mice.- In Vivo Bioluminescent Imaging of Marburg Virus in a Rodent Model.- Continuous and Real-time In Vivo Autobioluminescent Imaging in a Mouse Model.- In vivo Tracking of Tumor-derived Bioluminescent Extracellular Vesicles in Mice.- Bioluminescence Imaging in the Chick Chorioallantoic Membrane Assay.- Application of a cybLuc Aminoluciferin for Deep Tissue Bioluminescence Imaging in Rodent Models.- The Application of Single Cell Bioluminescent Imaging to Monitor Circadian Rhythms of Individual Plant Cells.


 
 

Notă biografică

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Textul de pe ultima copertă

This volume provides detailed protocols and methodologies required to perform bioluminescent imaging in multiple stages, enabling the reader to integrate this technology into their laboratory-based imaging experiments. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 Authoritative and cutting-edge, Bioluminescent Imaging: Methods and Protocols to ensure successful results in the further study of this vital field.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step details essential for reproducible results Contains key notes and implementation advice from the experts