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Adipose-Derived Stem Cells: Methods and Protocols de Bruce A. Bunnell

Adipose-Derived Stem Cells: Methods and Protocols: Methods in Molecular Biology, cartea 1773

Editat de Bruce A. Bunnell, Jeffrey M. Gimble
en Limba Engleză Hardback – 24 apr 2018
This second edition volume expands on the previous edition with a comprehensive update on recent developments in the Adipose Stromal/Stem Cells (ASC) field and the use of stroma vascular fraction (SVF) as a potential therapeutic strategy. The chapters in this book discuss protocols that are focused on discovery, preclinical, and clinical processes. Although most chapters focus on human ASC, there are several chapters that look at small and large animal species with pre-clinical relevance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips in troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Adipose-Derived Stem Cells: Methods and Protocols, Second Edition is a valuable resource for anyone interested in learning more aboutthe scientific advances in the field of ASC biology and application.

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Specificații

ISBN-13: 9781493977970
ISBN-10: 1493977970
Pagini: 305
Ilustrații: XII, 284 p. 56 illus., 46 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.82 kg
Ediția:2nd ed. 2018
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Isolation and Flow Cytometric Analysis of the Stromal Vascular Fraction Isolated from Mouse Adipose Tissue.- A Method for Isolation of Stromal Vascular Fraction Cells in a Clinically Relevant Timeframe.- Scaffold-Free, Size-Controlled Three-Dimensional Culture of Rabbit Adipose-Derived Stem Cells.- The Differentiation of Brown Adipocyte Progenitors Derived from Human Induced Pluripotent Stem Cells.- Methylcellulose Based Thermally Reversible Hydrogels.- Decellularized Adipose Tissue Scaffolds for Soft Tissue Regeneration and Adipose-Derived Stem/Stromal Cell Delivery.- Induction of Skin Allograft Transplantation Tolerance in Mice Using Human Adipose Derived Stromal Cells.- High Definition Confocal Imaging Modalities for the Characterization of Tissue-Engineered Substitutes.- Adipose-Derived Stromal Vascular Fraction Cells and Platelet-Rich Plasma: Basic and Clinical Implications for Tissue Engineering Therapies in Regenerative Surgery.- Myogenic Differentiation of ASCs using Biochemical and Biophysical Induction.- Isolation of Murine Adipose-Derived Stromal/Stem Cells for Adipogenic Differentiation or Flow Cytometry-Based Analysis.- Three-Dimensional Magnetic Levitation Culture System Simulating White Adipose Tissue.- Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates.- Isolation of Murine Adipose-Derived Stromal/Stem Cells using an Explant Culture Method.- Feline Adult Adipose Tissue-Derived Multipotent Stromal Cell Isolation and Differentiation.- Soft Tissue Reconstruction.- Mechanical Stimulation of Adipose-Derived Stem Cells for Functional Tissue Engineering of the Musculoskeletal System via Cyclic Hydrostatic Pressure, Simulated Microgravity, and Cyclic Tensile Strain.- Cryopreservation Protocols for Human Adipose Tissue Derived Adult Stem Cells.- Bone Regeneration with a Combination of Adipose-Derived Stem Cells and Platelet-Rich Plasma.- Isolationand Differentiation of Murine Primary Brown/Beige Preadipocytes.

Textul de pe ultima copertă

This second edition volume expands on the previous edition with a comprehensive update on recent developments in the Adipose Stromal/Stem Cells (ASC) field and the use of stroma vascular fraction (SVF) as a potential therapeutic strategy. The chapters in this book discuss protocols that are focused on discovery, preclinical, and clinical processes. Although most chapters focus on human ASC, there are several chapters that look at small and large animal species with pre-clinical relevance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips in troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Adipose-Derived Stem Cells: Methods and Protocols, Second Edition is a valuable resource for anyone interested in learning more aboutthe scientific advances in the field of ASC biology and application.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Recenzii

From the reviews:
“This book is pleasing the reader … . Clear enough, the statistics is impressive … . I am quite sure that the reader, after reading this unique and invaluable book, will easily share my idea that the ASC acronym, stending for Adipose-derived Stem/Stromal Cells and describing the collagenase-digested and culture-expanded adipose cell populations, will for sure appear more and more in the ISI statistics.” (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)