Extensive information regarding the topic of tandem mass spectrometry has been presented in this book. It provides in-depth elucidation on the theory, description as well as the instrumentation of experimental strategies and MS/MS data interpretation for the structural analysis of important molecular compounds. Analysis of carbohydrates, drugs, metabolites and protein post-translation modifications are the major topics described in this book. It aims to serve as a valuable reference for various audiences including graduate students, professionals engaged in this field as well as general readers interested in the use of modern mass spectrometry for getting answers to crucial questions of prime importance in biological and chemical sciences.