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Small Non-Coding RNAs: Methods and Protocols de Mathieu Rederstorff

Small Non-Coding RNAs: Methods and Protocols: Methods in Molecular Biology, cartea 2300

Editat de Mathieu Rederstorff
en Limba Engleză Paperback – 2 apr 2022
This second edition provides new and updated chapters detailing methods tackling all aspects of small non-coding RNAs biology.  Chapters guide readers through customized dedicated protocols and technologies that will be of valuable help decipher the numerous functions of small non-coding RNAs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on  troubleshooting and avoiding known pitfalls.
Instructive and practical, Small Non-Coding RNAs: Methods and Protocols, Second Edition aims to be a valuable help and of great interest to a large number of researchers working in the field of RNA or willing to develop RNA studies.
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Specificații

ISBN-13: 9781071613887
ISBN-10: 107161388X
Pagini: 270
Ilustrații: X, 270 p. 66 illus., 48 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.49 kg
Ediția:2nd ed. 2021
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Regulatory Non-coding RNAs: An Overview.- RNA Precipitation.- Quantitative and Qualitative Assessment of Small RNA Preparations.- Non-coding RNA Silencing in Mammalian Cells by Antisense LNA GapmeRs Transfection.- Northern Blot Detection of Tiny RNAs.- Stem-loop qRT-PCR Based Quantification of miRNAs.- Detection and Labeling of Small Non-coding RNAs by Splinted Ligation.- Fluorescence In Situ Hybridization Of Small Non-coding RNAs.- Using native RIP, UV-CLIP or fCLIP to Address Protein-RNA Interactions In Vivo.- Purification of RiboNucleoProtein Particles by MS2-MBP Affinity Chromatography.- Study of genome-wide occupancy of long non-coding RNAs using Chromatin Isolation by RNA Purification (ChIRP).- Gene Reporter Assays to Study miRNA Function.- CRISPR/Cas9 System to Knock-Down microRNA In Vitro and In Vivo.- Microarray Analysis of Whole-Transcriptome RNAs including Non-Coding RNAs.- Isolation, Extraction, and Deep-Sequencing Analysis Of Extracellular RNAs (exRNAs) from Human Plasma.- Analysis of the RNA and protein complexome by Grad-seq.- µIVC-Seq: A Method for Ultrahigh-throughput Development and Functional Characterization of Small RNAs.- RNA Secondary Structure Study by Chemical Probing Methods using DMS and CMCT.- Application of NMR Spectroscopy to Determine the 3D structure of Small Non-coding RNAs.

Textul de pe ultima copertă

This second edition provides new and updated chapters detailing methods tackling all aspects of small non-coding RNAs biology.  Chapters guide readers through customized dedicated protocols and technologies that will help decipher the numerous functions of small non-coding RNAs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.
Instructive and practical, Small Non-Coding RNAs: Methods and Protocols, Second Edition aims to be a valuable help and of great interest to a large number of researchers working in the field of RNA or willing to develop RNA studies.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Notă biografică

Mathieu Rederstorff, PhD Associate Professor Laboratoire IMoPA UMR 7365 CNRS/UL Equipe ARN, RNP - Maturation, Structure, Fonction - Université de Lorraine Biopôle/Campus Médecine Brabois 9 Avenue de la Forêt de Haye BP 184 54506 Vandoeuvre-lès-Nancy FRANCE Tel: +33 3 83 68 55 31 (office) +33 3 83 68 55 25 (lab) +33 6 49 12 67 11 (mobile) Fax: +33 3 83 68 55 09 (fax) mathieu.rederstorff@univ-lorraine.fr mathieu.rederstorff@maem.uhp-nancy.fr