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Selective Autophagy: Methods and Protocols de Ioannis P. Nezis

Selective Autophagy: Methods and Protocols: Methods in Molecular Biology, cartea 2845

Editat de Ioannis P. Nezis
en Limba Engleză Hardback – 17 sep 2024
This volume presents the latest techniques used by researchers to study selective autophagy in a broad range of organisms such as yeast, plants, worms, fruit flies, and mammals. The chapters in this book cover several types of selective autophagy such as mitophagy, aggrephagy, ER-phagy, ferritinophagy, pexophagy, and nucleophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Selective Autophagy: Methods and Protocols is a valuable resource for novice and expert researchers who want to learn more about this interesting and developing field.

Chapter 3 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
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Specificații

ISBN-13: 9781071640661
ISBN-10: 1071640666
Pagini: 260
Ilustrații: CCLXX, 270 p. 53 illus., 42 illus. in color.
Dimensiuni: 178 x 254 x 20 mm
Greutate: 0.67 kg
Ediția:2024
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Using Fluorescence Microscopy and Immunoblotting for Monitoring Mitophagy in Budding Yeast.- Methods to Monitor Nucleophagy in Yeast.- Synthetic Dimerization Approaches for In Vivo Studies in Saccharomyces cerevisiae.- Assays to Study Mitophagy in Plants.- Assessing Neuronal Mitophagy during Development in the Nematode Caenorhabditis elegans.- In Vivo Monitoring of Nucleophagy in Caenorhabditis elegans.- Analysis of Mitophagy Reporters in Drosophila.- Studying Selective Autophagy of Protein Aggregates using Particles Induced by Multimerization (PIMs).- Methods for Monitoring ER-phagy.- Quantitative Super-Resolution Imaging of ER-phagy Initiation in Cells.- Fluorescence Methods to Measure Pexophagy.- Methods for Monitoring Mitophagy using mt-Keima.- Monitoring Mitophagy Dynamics in Live Cells.- Methods for Monitoring NCOA4-Mediated Ferritinophagy.- Method to Purify Phase-Separated p62 Bodies using Fluorescence-Activated Particle Sorting.- In Silico Assessment of LIR Motif-Containing Proteins Binding to ATG8-Family Proteins.- High Throughput Screening for LC3/GABARAP Binders Utilizing the Fluorescence Polarization Assay.- Thermodynamic Characterization of LC3/GABARAP:Ligand Interactions by Isothermal Titration Calorimetry.- Determination of hATG8 Binding Selectivity of Autophagy-Interacting Motif (AIM) Peptides Using Native Electrospray Ionization Mass Spectrometry.

Textul de pe ultima copertă

This volume presents the latest techniques used by researchers to study selective autophagy in a broad range of organisms such as yeast, plants, worms, fruit flies, and mammals. The chapters in this book cover several types of selective autophagy such as mitophagy, aggrephagy, ER-phagy, ferritinophagy, pexophagy, and nucleophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Selective Autophagy: Methods and Protocols is a valuable resource for novice and expert researchers who want to learn more about this interesting and developing field.

Chapter 3 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts