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RNA Labeling de Je-Hyun Yoon

RNA Labeling: Methods in Molecular Biology, cartea 3047

Editat de Je-Hyun Yoon
en Limba Engleză Hardback – 3 sep 2026
This volume provides comprehensive methods and protocols to detect and trace RNAs in biochemistry, molecular, cell biology, genetics, genomics of RNA biology. Chapters detail RNA labeling, RNA biology of mammalian cells, and methods on model organisms; including Zebrafish, Drosophila, C. elegans, budding yeast as well as prokaryotes including E. coli. Written for the highly successful Methods in Molecular Biology series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
 
Authoritative and up-to-date, RNA Labeling: Methods and Protocols serves as a vital resource for researchers working in RNA labeling.
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Specificații

ISBN-13: 9781071653517
ISBN-10: 1071653512
Pagini: 272
Dimensiuni: 183 x 260 x 21 mm
Greutate: 0.71 kg
Editura: Springer Us
Colecția Methods in Molecular Biology
Seria Methods in Molecular Biology

Cuprins

Labeling of Northern Blot Probes.- Complementary Approaches for Accurate Detection of tRNA-Derived Small RNAs (tsRNAs): Small RNA Northern Blotting and Dumbbell PCR.- RNA labeling for measuring RNA endonucleolytic cleavage.- Molecular analysis of miRNA stability and decay dynamics by northern blot.- RNA labeling with Fluorophore.- Labeling of Amine-Modified RNA.- smFISH: Quantifying and Mapping RNA at the Single-Molecule Level.- Single-molecule RNA fluorescence in situ hybridization (smRNA-FISH) labeling and imaging of mRNA to determine subcellular localization.- RNA Labeling of Nucleolar Transcripts by 5-ethynyl uridine Click Chemistry.- Simultaneous Visualization of NEAT1_2 and Paraspeckle Proteins by Sequential RNA-FISH and Immunofluorescence.- Detection of long non-coding RNA localization by RNA fluorescence in situ hybridization (FISH).- Labeling of Long Noncoding RNAs to Study Virus-Induced Nuclear Aggregates.- RBProximity-CLIP: A Method for Profiling the Interactome of an RNA Binding Protein at Subcellular resolution.- RAP-RNA: A Methodological Approach to Study Base-Pairing Between Endogenous RNAs.- Assay for in-vitro arginine methylation of RNA binding proteins followed by RNA binding.- Fluorescence-based mRNA translation reporter.- 5EU-Based Tracking of RNA Transfer through Extracellular Vesicles.- Analysis of endogenously expressed double-stranded RNAs in human cultured cells.