Regulatory Protein Modification

Analysis of Protein Phosphorylation in Intact Cells and Extracts.- Analysis of Protein Dephosphorylation in Intact Cells and Extracts.- Protein Phosphorylation and Dephosphorylation in Isolated Nerve Terminals (Synaptosomes).- Protein Kinase and Phosphatase Inhibitors.- Phosphorylation State-Specific Antibodies.- Protein Tyrosine Phosphorylation.- Identification of Posttranslational Modification Sites by Site-Directed Mutagenesis.- Protein methylation in the nervous system.- Long-Chain Fatty Acylation of Proteins.- Protein ADP-Ribosylation.- Glycosylation and Glycosylphosphatidylinositol Membrane Anchors.

Hugh Hemmings' Regulatory Protein Modification: Techniques and Protocols offers critical reviews of major topics concerning posttranslational protein modification in neurons, as well as a comprehensive collection of state-of-the-art techniques for their analysis. Its distinguished international contributors provide detailed and specific methods for studying protein phosphorylation/dephospohorylation, methylation, long chain fatty acylation, ADP-ribosylation, glycosylation, and glycosylphosphatidylinositol anchors. Using both conventional analytical and novel molecular biological approaches, these experts discuss the techniques and reagents and cite numerous examples from the neuroscience literature in a way that allows investigators to determine the best experimental approaches for their own systems. They also
offer a complete review of protein kinase and phosphatase inhibitors techniques and their applications today.

Regulatory Protein Modification: Techniques and Protocols will prove an exceptionally valuable resource for both experienced and new investigators working in neuronal and nonneuronal systems.