Aquatic invertebrates are an important biomonitoring tool for understanding and detecting changes in ecosystem integrity over time. Therefore, the monitoring objectives of this protocol as described by DeBacker et al. (2005) are: 1) Determine the status and trends of invertebrate species diversity, abundance, and community metrics. 2) Relate invertebrate community to overall water quality through quantification of metrics related to species richness, abundance, diversity, and region-specific multi-metric indices as indicators of water quality and habitat condition.