Prokaryotic Genomics: Methods and Tools in Biosciences and Medicine

1 Physical Analysis of Chromosome Size Variation.- 2 Genetic Mapping in Salmonella enterica.- 3 Insertion Sequences as Genomic Markers.- 4 The Use of Noncoding Microsatellite Length Analysis for Bacterial Strain Typing.- 5 How to Amplify Easily, on the Bacterial Chromosome, a Desired DNA Sequence.- 6 Generalized Transduction.- 7 Use of Conditional-replication, Integration, and Modular CRIM Plasmids to Make Single-copy lacZ Fusions.- 8 Genetic Footprinting for Bacterial Functional Genomics.- 9 Gene Transfer to Plants through Bacterial Vectors.- 10 Quorum Sensing: Approaches to Identify Signals and Signalling Genes in Gram-negative Bacteria.- 11 Transcriptional Profiling in Bacteria Using Microarrays.- 12 Transcriptome Analysis by Macroarrays.- 13 Prokaryotic Proteomics.- 14 Intein-mediated Protein Purification.- 15 Two-hybrid Assay in Escherichia coli K12.- Guide to Protocols.

THE BOOK DESCRIBES IMPORTANT TECHNIQUES FROM MODERN PROKARYOTIC GENETICS AND GENOMICS WITH EXTENSION TO METHODS FROM PROTEOMICS MIXTURE BETWEEN IMPORTANT ESTABLISHED AND TOPICAL METHODS NO RIVAL METHOLOGY BOOK KNOWN THAT COVERS PROKARYOTIC GENETICS, GENOMICS AND PROTEOMICS AN EMBO COURS ORGANIZED BY PROF. BLOT ON PROKARYOTIC GENETICS TODAY SERVED AS A BASIS FOR THE BOOK (EMBO =3D EUROPEAN MOLECULAR BIOLOGY ORGANIZAT