Stem cells have developed a great amount of excitement among the clinicians, researchers and the public alike. Several types of stem cells are being examined for their regenerative ability. Slight advantage because of transplanting autologous stem cells in several distinct clinical conditions has been suggested to be a progress factor effect rather than actual regeneration. In comparison, several pre-clinical analyses have been carried out, with the help of differentiated cells from induced pluripotent stem cells or embryonic stem cells have displayed functional advancement, promise and zero signs of teratoma formation. This book is a compilation of studies/reviews, starting with an introduction to the pluripotent stem cells and encompassing aspects like differentiation and derivation under two broad sections namely, pluripotent stem cells: genesis & means and mechanistic underpinning. These topics include the generation of cells and diverse ways of their derivation from adult as well as embryonic tissues, and the mechanistic comprehension of pluripotency along with the related pros and cons in the same are covered in this book.