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Plant Epigenetics: Methods and Protocols de Igor Kovalchuk

Plant Epigenetics: Methods and Protocols: Methods in Molecular Biology, cartea 1456

Editat de Igor Kovalchuk
en Limba Engleză Paperback – 7 iul 2018
Thisvolume provides a variety of protocols to analyze various epigeneticchanges, including differential expression of non-coding RNAs, changes in DNAmethylation, and histone modifications in plants. Chapters detail protocolswith different degrees of complexity, and describe bioinformatics approachesfor data processing and analysis. Written in the highly successful Methodsin Molecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.
Authoritative andcutting-edge, Plant Epigenetics: Methods and Protocols, Second Editionaims to ensure successful results in the further study of this vital field.


 
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Specificații

ISBN-13: 9781493979561
ISBN-10: 1493979566
Pagini: 253
Ilustrații: X, 253 p. 51 illus., 32 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.46 kg
Ediția:Softcover reprint of the original 2nd ed. 2017
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Chromatin ImmunoprecipitationProtocol for Histone Modifications and Protein-DNA Binding Analyses in Arabidopsis.- Chromatin Conformation Capture-Based Analysis of Nuclear Architecture.- Metaanalysis of Genome-Wide Chromatin Data.- Localization of Mirnas by In Situ Hybridization in Plants using ConventionalOligonucleotide Probes.- COBRA (Combined Bisulfite Restriction Analysis) Assay for the Analysis ofLocus-Specific Changes in Methylation Patterns.- Analysis of Global Genome Methylation Using the Cytosine-Extension Assay.- In Situ Analysis Of DNA Methylation In Plants.- Analysis of DNA Hydroxymethylation In Arabidopsis UsingColorimetric Assay.- Analysis of DNA Cytosine Methylation Patterns usingMethylation Specific Amplification Polymorphism (MSAP).- Differentially MethylatedRegion - Representational Difference Analysis (DMR-RDA): A Powerful Method to IdentifyDmrs in Uncharacterized Genomes.- Analysis of Small RNA PopulationsUsingHybridization to DNA Tiling Arrays.- Northern Blotting Techniques for Small RNAs.- Stem-Loop Qrt-PCR for the Detection of PlantMicrornas.- Profiling New Small RNA Sequences.- Small RNA Library Preparation and Illumina Sequencing in Plants.- Bioinformatics Analysis of Small RNA Transcriptomes - The Detailed Work Flow.- Increasinga Stable Transformation Efficiency of Arabidopsis by Manipulating theEndogenous Gene Expression using Virus-Induced Gene Silencing.- The Random Oligonucleotide-Primed Synthesis Assay for the Quantification of DNAStrand Breaks.- Profiling Transposable Elements and their Epigenetic Effects in Non-ModelSpecies.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts