Peroxisomes: Springer Lab Manuals

I: Isolation and Characterization of Peroxisomes.- General Indroduction to Isolation and Characterization of Peroxisomes.- Experiment 1. Preparation and Purification of Peroxisomes; Subfractionation of Purified Peroxisomes; Acyl-CoA Oxidase Activity Measurements.- Experiment 2. Immunoblotting of Peroxisomal Proteins with Monospecific Antibodies.- Experiment 3. Morphology of Peroxisomes in Light- and Electron Microscopy.- II: Molecular Biology.- Experiment 4. Northern Blotting Analysis of Rat Liver mRNA Encoding for Peroxisomal Acyl-CoA Oxidase.- Experiment 5. Transient Co-transfection Assay: Measure of the Chloramphenicol Acetyl Transferase (CAT) Activity in Cytosol Extracts from Transfected Cells.- Experiment 6. Synthesis of Oligonucleotides Used as Probes; Purification by HPLC.- Experiment 7. Computer Analysis of DNA and Protein Sequences.- III: Toxicology and Pharmacology.- Experiment 8. Cytochrome P450 Isoenzyme Induction Pattern in Rats Treated with Peroxisome Proliferators and Classical Inducers.- Experiment 9. Measurement of the in Vitro Glucuronidation of Peroxisome Proliferator Carboxylic Acids by Liver Microsomes and by Genetically Modified V 79 Cell Line.- Experiment 10. Molecular Modeling and Drug Design: Application to Some Peroxisome Proliferator Agents.- IV: Cell Culture and Genetic Diseases.- Experiment 11. Fao Cell Line as a Model for the Study of the Effect of Peroxisome Proliferators on Cellular Functions.- Experiment 12. Measurement of Dihydroxyacetone-Phosphate Acyl-Transferase (DHAP-AT) Activity in Liver Peroxisomes and Cell Lines.- Experiment 13. In Vitro Transformation of Human Fibroblasts with SV40 T Antigen (Lipofection).- Experiment 14. Somatic Cell Hybridization as a Tool for Genetic Analysis of Peroxisomal Activities.- Supplement.