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Basic Cloning Procedures de Valdis Berzins

Basic Cloning Procedures: Springer Lab Manuals

Editat de Valdis Berzins
en Limba Engleză Paperback – 7 iul 2012

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Specificații

ISBN-13: 9783642489778
ISBN-10: 364248977X
Pagini: 180
Ilustrații: VII, 169 p.
Dimensiuni: 155 x 235 x 9 mm
Greutate: 0.26 kg
Ediția:1998
Editura: Springer Berlin, Heidelberg
Colecția Springer
Seria Springer Lab Manuals

Locul publicării:Berlin, Heidelberg, Germany

Public țintă

Research

Cuprins

1 cDNA Synthesis and Cloning.- 2 DNA Sequencing.- 3 RNA In Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase.- 4 PCR-Based Site-Specific Mutagenesis.- 5 Analysis of Specific Protein-DNA Interactions.- 6 Gel Electrophoresis, Transfer and Hybridization of Nucleic Acids.- 7 Immunological Methods for Analysis of Recombinant Proteins.- 8 Protein Synthesis in Cell-Free Systems.

Textul de pe ultima copertă

This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints.

Caracteristici

Basic laboratory techniques, approved in several international molecular biology courses Step-by-step protocols with detailed practical hints, troubleshooting guide and short background information The protocols are written by authors with intensive practical experience