Oligodendrocytes
Editat de Ebru Ercan Herbsten Limba Engleză Hardback – 16 noi 2026
This volume provides comprehensive collection of contemporary protocols spanning the broad methodological landscape of oligodendrocyte research.Chapters detail genetic manipulation, functional assays, and the study of oligodendrocytes in disease models and therapeutic contexts. Written for the highly successfulMethods in Molecular Biologyseries, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and practical,Oligodendrocytes: Methods and Protocolsaims to be an invaluable guide for novices and experienced scientists, to advance the understanding of oligodendrocyte biology.
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Specificații
Cuprins
Isolation and culture of oligodendrocyte lineage cells from rodent brains.-Purification of oligodendrocyte precursor cells from neonatal rodent CNS by immunopanning.-Directed differentiation of iPSCs into oligodendrocyte and astrocyte enriched cultures.-Transitions from rodent primary cultures to human stem cell derived systems.-Selective transduction of primary oligodendrocytes using a lentivirus approach.-In vitro myelination using DRG explants an efficient model for quantitative evaluation of peripheral nerve myelination in culture.-Myelinating co cultures with CNS derived axons and analysis of myelin formation with live imaging.-Measuring conduction velocity by compound action potential (CAP) recordings.-Cuprizone Induced Demyelination and Remyelination Models of Multiple Sclerosis.- Analysis of myelination and oligodendrocytes in Zebrafish.-Single and dual colour in vivo Ca2+ imaging in oligodendrocyte precursor cells and neurons in zebrafish.-Metabolic Imaging of Optic Nerve Oligodendrocytes Following AAV Mediated Sensor Delivery.- Immunohistochemical analysis of oligodendrocyte lineage cells and myelin in murine central nervous system.-Visualization and Morphological Analysis of Individual Oligodendrocytes in the Mouse White Matter.-Blocks pins and heavy metal optimized sample preparation for volume EM of myelin in mouse central nervous system.-Isolation of oligodendrogial nuclear RNA from fresh frozen human brains for transcriptomic analysis.- Advanced diffusion MRI in the human brain microstructure imaging and structural connectivity.